MABC1609M

Sigma-Aldrich

Anti-AIF Antibody, clone 3C11

clone 3C11, from mouse

别名:
Apoptosis-inducing factor 1, mitochondrial, Programmed cell death protein 8
eCl@ss:
32160702
价格与库存信息目前不能提供

Quality Level

biological source

mouse

antibody product type

primary antibodies

clone

3C11, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

application(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

ambient

General description

Apoptosis-inducing factor 1, mitochondrial (UniProt: O95831; also known as Programmed cell death protein 8) is encoded by the AIFM1 (also known as AIF, PDCD8) gene (Gene ID: 9131) in human. AIF is a nuclear encoded flavoprotein that is generally confined to the mitochondrial intermembrane space and functions both as NADH oxidoreductase and as regulator of apoptosis. Six isoformsi of AIF have been described that are produced by alternative splicing. Under normal conditions, a 54-residue N-terminal (transit peptide) segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase to form the inner-membrane-anchored mature form (AIFmit). Upon induction of apoptosis, it is further proteolytically processed at to generate the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner. AIF also functions as an anti-apoptotic factor in normal mitochondria via its NADH oxidoreductase activity. Once released from mitochondria it translocates to the nucleus to induce caspase-independent fragmentation of chromosomal DNA. Mutations in AIF gene are linked to combined oxidative phosphorylation deficiency 6 that is characterized by psychomotor delay, hypotonia, areflexia, and muscle weakness and wasting. Defects in this gene are also known to cause Cowchock syndrome that leads to early childhood onset of a slowly progressive axonal sensorimotor neuropathy.

Specificity

Clone 3C11 specificallly detects AIF in human cells.

Immunogen

His-tagged full length recombinant human AIF.

Application

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected AIF in human skin, human testis, and human kidney tissue.
Anti-AIF, clone 3C11, Cat. No. MABC1609, is a mouse monoclonal antibody that detects AIF and has been tested for use in Immunohistochemistry (Paraffin), and Western Blotting.
Research Category
Apoptosis & Cancer

Quality

Evaluated by Western Blotting in A431 cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected AIF in 10 µg of A431 cell lysates.

Target description

~67 kDa observed; 66.90 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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