MABC1691M

Sigma-Aldrich

Anti-BLUE Antibody, clone 2D2-F11

clone 2D2-F11, from mouse

eCl@ss:
32160702
价格与库存信息目前不能提供

Quality Level

biological source

mouse

antibody product type

primary antibodies

clone

2D2-F11, monoclonal

species reactivity (predicted by homology)

all

packaging

antibody small pack of 25 μg

application(s)

western blot: suitable

isotype

IgG1κ

General description

Pre-stained proteins are commonly used as molecular weight markers in electrophoresis. Several protein markers are available as pre-stained proteins with vinyl sulfone dyes. After separation through electrophoresis, the protein of interest is visualized by specific antibody coupled to a fluorophore or by chemiluminescence detection system. Anti-Blue antibody, Clone 2D2-F11, is a unique antibody that can detect pre-stained molecular markers from different vendors without the need to perform a manual marking and estimating molecular weights. The use of this antibody allows for visualization of marker proteins along with the protein of interest on the same blot. Anti-Blue antibody offers high specificity and does not interfere with the detection of other antibodies, In addition, it does not display reactivity with other cellular proteins, unstained marker proteins, or with proteins stained with different dyes.

Specificity

Anti-BLUE, clone 2D2-F11, is a mouse monoclonal antibody that detects prestained Precision Plus Protein marker proteins.

Immunogen

Prestained Precision Plus Protein marker proteins.

Application

Research Category
Secondary & Control Antibodies
Anti-BLUE, clone 2D2-F11, is a mouse monoclonal antibody that detects prestained Precision Plus Protein marker proteins and has been tested for use in Western Blotting.
Western Blotting Analysis: A representative lot detected BLUE in Western Blotting applications (Schuchner, S., et. al. (2016). Sci Rep. 6:31363).

Quality

Evaluated by Western Blotting in Pre-Stained Protein Standard.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected BLUE-stained marker protein standards.

Physical form

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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