Merck
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MABC1765

Sigma-Aldrich

Anti-TfR1 (CD71) Antibody

purified antibody, clone 3F3-FMA, from mouse

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生物来源

mouse

质量水平

抗体形式

purified antibody

antibody product type

primary antibodies

克隆

3F3-FMA, monoclonal

分子量

calculated mol wt 84.87 kDa
observed mol wt ~90 kDa

species reactivity

human, mouse

包装

antibody small pack of 100 μL

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

同位素/亚型

IgG

运输

dry ice

储存温度

2-8°C

target post-translational modification

unmodified

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此商品
ZRB1412ZMS1031ZMS1041
antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

3F3-FMA, monoclonal

clone

recombinant monoclonal

clone

monoclonal, recombinant monoclonal

clone

recombinant monoclonal

mol wt

calculated mol wt 84.87 kDa, observed mol wt ~90 kDa

mol wt

calculated mol wt 30.82 kDa, observed mol wt ~35 kDa

mol wt

calculated mol wt 217.18 kDa, observed mol wt ~300 kDa

mol wt

calculated mol wt 55.23 kDa, observed mol wt ~75 kDa

species reactivity

human, mouse

species reactivity

mouse, human

species reactivity

human

species reactivity

human

packaging

antibody small pack of 100 μL

packaging

antibody small pack of 25 μL

packaging

antibody small pack of 25 μL

packaging

antibody small pack of 25 μL

特异性

Clone 3F3-FMA is a mouse monoclonal antibody that detects human Transferrin receptor protein 1.

免疫原

Purified membrane fractions from OCI-LY7 cells.

应用

Quality Control TestingEvaluated by Western Blotting in HT-1080 cell lysate.Western Blotting Analysis: A 1:500 dilution of this antibody detected TfR1 (CD71) in HT-1080 cell lysate.Tested ApplicationsFlow Cytometry Analysis: A representative lot detected TfR1 (CD71) in Flow Cytometry applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TfR1 (CD71) in HT-1080 cells.Immunohistochemistry Applications: A representative lot detected TfR1 (CD71) in Immunohistochemistry applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423).Immunocytochemistry Analysis: A representative lot detected TfR1 (CD71) in Immunocytochemistry applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423). Western Blotting Analysis: A representative lot detected TfR1 (CD71) in Western Blotting applications (Feng, H., et. al. (2020). Cell Rep. 30(10): 3411-3423). Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-TfR1 (CD71), clone 3F3-FMA, Cat. No. MABC1765, is a mouse monoclonal antibody that detects Tfr1 and is an excellent ferroptosis marker. It is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

目标描述

Transferrin receptor protein 1 (UniProt: Q62351; also known as TR, TfR, TfR1, Trfr, T9, p90, CD71) is encoded by the Tfrc (also known as Trfr) gene (Gene ID: 7037) in human. TfR1 is a single-pass type II membrane glycoprotein with a cytoplasmic domain (aa 1-67), a transmembrane domain (aa 68-88), and a long extracellular domain (aa 89-760). It can also be proteolytically cleaved at Arg100 to generate the soluble serum form (sTfR). TfR1 is involved in the uptake of iron via receptor mediated endocytosis into specialized endosomes where acidification leads to release of iron. TfR1 is reported to be essential for erythrocyte development and positive regulation of T and B cell proliferation through iron uptake. It also acts as a lipid sensor that regulates mitochondrial fusion by regulating the activation of the JNK pathway and degradation of mitofusin MFN2. Under conditions of low dietary levels of stearate (C18:0), it promotes the activation of JNK pathway that results in HUWE1-mediated ubiquitination and degradation of mitofusin MFN2 and inhibition of mitochondrial fusion. Clone 3F3-FMA is reported to be an effective ferroptosis-staining agent. It stains plasma membrane and the perinuclear region associated with the Golgi and the endosomal recycling compartment. (Ref.: Senilymaz, D., et al. (2015). Nature. 525(7567); 124-128; Feng, H., et al. (2020). Cell Rep. 30(10); 3411-3423).

外形

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at +2°C to +8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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