MABE1932

Sigma-Aldrich

Anti-EWS Antibody, clone 21B1

clone 21B1, from rat

别名:
RNA-binding protein EWS, EWS oncogene, Ewing sarcoma breakpoint region 1 protein
eCl@ss:
32160702
价格与库存信息目前不能提供

biological source

rat

antibody product type

primary antibodies

clone

21B1, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μL

application(s)

ELISA: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

General description

RNA-binding protein EWS (UniProt: Q01844; also known as EWS oncogene, Ewing sarcoma breakpoint region 1 protein) is encoded by the EWSR1 (also known as EWS) gene (Gene ID: 2130) in human. EWS is member of the TET family of DNA and RNA-binding proteins, which also includes translocated in liposarcoma/fused in sarcoma protein (FUS/TLS) and TATA-binding protein-associated factor 15. It is a multifaceted RNA binding protein with established roles in transcription, pre-mRNA processing, and DNA damage response. It contains a transcriptional-activation domain (EAD), 3 glycine-arginine rich regions, an RNA-binding domain, and a zinc finger domain (aa 518-549). EWS is highly methylated on arginine residues and methylation is mediated by PRMT1 and to some extent by PRMT8. Various environmental signals are known to induce post-translational modifications in its RNA binding domain and glycine-arginine rich domains, thus modulating EWS activity. EWS can relocate from cytoplasm to ribosomes upon PTK2B/FAK2 activation. Mutations in EWS gene are known to cause Ewing sarcoma, a highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue.

Specificity

Clone 21B1 is a rat monoclonal antibody that detects human RNA-binding protein EWS. It targets an epitope with in 20 amino acids from the C-terminal region.

Immunogen

Ovalbumin-conjugated linear peptide corresponding to 20 amino acids from the RGG3 domain of human RNA-binding protein EWS.

Application

Anti-EWS, clone 21B1, Cat. No. MABE1932, is a highly specific rat monoclonal antibody that targets RNA-binding protein EWS and has been tested in ELISA and Western Blotting.
ELISA Analysis: A representative lot detected EWS in ELISA applications (Suarez-Calvet, M, et. al. (2016). Acta Neropathol. 131(4):587-604).

Western Blotting Analysis: A representative lot detected EWS in Western Blotting applications (Suarez-Calvet, M, et. al. (2016). Acta Neropathol. 131(4):587-604).
Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected EWS in HeLa cell lysate.

Target description

~85 kDa observed; 68.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Protein G purified
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Marc Suárez-Calvet et al.
Acta neuropathologica, 131(4), 587-604 (2016-02-20)
Deposition of the nuclear DNA/RNA-binding protein Fused in sarcoma (FUS) in cytosolic inclusions is a common hallmark of some cases of frontotemporal lobar degeneration (FTLD-FUS) and amyotrophic lateral sclerosis (ALS-FUS). Whether both diseases also share common pathological mechanisms is currently...

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