MABF156C

Anti-CD4 (Mouse), APC, clone RM4-5 Antibody

clone RM4-5, from rat, Allophycocyanin conjugate

• Synonym(s): T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, CD4, CD4
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• 生物来源: rat
• 质量水平: 100
• 偶联物: Allophycocyanin conjugate
• 抗体形式: purified antibody
• antibody product type: primary antibodies
• 克隆: RM4-5, monoclonal
• species reactivity: mouse
• 包装: antibody small pack of 25 μg
• technique(s): flow cytometry: suitable
• 同位素/亚型: IgG2aκ
• UniProt登记号: P06332
• Gene Information: mouse ... Cd4(12504)

Description

一般描述

The RM4-5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will “compete” for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.

免疫原

Immunogen information for this antibody is proprietary information.

应用

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Anti-CD4 (Mouse), APC, clone RM4-5 Antibody is validated for use in Flow Cytometry for the detection of CD4.

质量

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD4 in one million C57Bl/6 splenocytes.

外形

Purified by size-exclusion chromatography

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: nwg
• 闪点(F): Not applicable
• 闪点(C): Not applicable