MABF2038

Sigma-Aldrich

Anti-Rabies virus Antibody, clone 802

clone 802, from mouse

别名:
Glycoprotein G Rabies Virus
eCl@ss:
32160702
NACRES:
NA.41
价格与库存信息目前不能提供

Quality Level

biological source

mouse

antibody product type

primary antibodies

clone

802, monoclonal

species reactivity

rabies virus

species reactivity (predicted by homology)

all

packaging

antibody small pack of 25 μg

application(s)

immunohistochemistry: suitable

isotype

IgG2bκ

UniProt accession no.

shipped in

ambient

General description

Glycoprotein (UniProt: P03524) of Rabies virus of Evelyn-Rokitnicki-Abelseth (ERA) strain is encoded by the G gene. Rabies virus belongs to the Lyssavirus serogroup of viruses. The glycoprotein of Rabies virus is a homotrimer, single-pass type I membrane protein that attaches the virus to host cellular receptor and thereby inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. It has been shown that the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and facilitate Rabies virus entry into cells. Arginine 352 is considered to be highly involved in Rabies virus pathogenicity and a mutation at this site dramatically attenuates the virus. Rabies virus glycoprotein is synthesized with a signal peptide (aa 1-19) that is subsequently cleaved off. It has two topological domains (aa 20-459 virion surface; 481-524 intravirion) and a helical domain (aa 460-480). Glycosylation of viral glycoprotein is considered to be crucial for glycoprotein export at the cell surface. Clone 802 is shown to recognize most Rabies virus strains, however it does not display neutralizing activity. (Ref.: Schumacher, CL et al. (1989). J. Clin. Invest. 84(3); 971-975; Bunschoten, H et al. (1989). J. Gen. Virol. 70(2); 291-298).

Specificity

Clone 802 detects Glycoprotein of Rabies virus (strain ERA). This clone does not display neutralizing activity in vitro.

Immunogen

Beta-propiolactone-inactivated Rabies Virus (ERA strain).

Application

This mouse monoclonal Anti-Rabies virus, clone 802, Cat. No. MABF2038, is a mouse monoclonal antibody that detects glycoprotein of Rabies virus and has been tested for use in Immunohistochemistry.
Immunohistochemistry Analysis: A representative lot detected Rabies virus in CVS-24 infected mouse and normal mouse tissues (Courtesy of the Ertl Lab, Wistar Institute, Philadelphia, PA USA).
Research Category
Inflammation & Immunology

Quality

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b.

Target description

58.66 kDa calculated.

Physical form

Protein G purified
Purified mouse monoclonal antibody IgG2b in PBS without preservatives.
Format: Purified

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Flash Point(F)

does not flash

Flash Point(C)

does not flash

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门

社交媒体

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

科研、开发、生产。

作为生命科学行业的全球领先供应商,我们致力于为科研、生物技术开发和生产,以及制药药物疗法开发和生产提供各类解决方案和服务。

© 2021年版权归德国达姆施塔特默克集团(Merck KGaA)及/或其附属公司所有。版权所有。

未经许可,严禁复制本网站上的任何资料。