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MABF2159

Sigma-Aldrich

Anti-CD63 (LAMP3) Antibody, clone ME491

clone ME491, from mouse

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别名:
CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30
eCl@ss:
32160702

生物来源

mouse

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

ME491, monoclonal

species reactivity

human

包装

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

同位素/亚型

IgG1κ

NCBI登记号

UniProt登记号

target post-translational modification

unmodified

Gene Information

human ... CD63(967)

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vibrant-m

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单克隆抗-CD63 小鼠抗

vibrant-m

SAB4301607

抗 CD63

Gene Information

human ... CD63(967)

Gene Information

human ... CD63(967)

Gene Information

human ... CD63(967)

Gene Information

human ... CD63(967)

clone

ME491, monoclonal

clone

polyclonal

clone

MEM-259, monoclonal

clone

polyclonal

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

rabbit

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

NCBI accession no.

NP_001244318

NCBI accession no.

-

NCBI accession no.

NM_001040034.1

NCBI accession no.

-

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一般描述

CD63 antigen (UniProt: P08962; also known as Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63) is encoded by the CD63 (also known as MLA1, TSPAN30) gene (Gene ID: 967) in human. CD63 is a multi-pass membrane protein of the tetraspan family that is found on endosome, lysosome, and plasma membranes. CD63 has been detected in platelets, Dysplastic nevi benign moles), radial growth phase primary melanomas, hematopoietic cells, and in tissue macrophages. In melanoma cells it is involved in their motility and adhesion. CD63 also plays a role in the adhesion of leukocytes onto endothelial cells. It is reported to play a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases and promote cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration. CD63 is a highly N-glycosylated protein with three asparagine glycosylation sites (aa 130, 150, 172) and its ribophorin II (RPN2)-mediated glycosylation has been linked to breast cancer. Overexpression of CD63 has been observed in esophageal cancer that is negatively correlated with tumor stage and lymph node metastasis. Lack of expression of CD63 in platelets has been observed in a patient with Hermansky-Pudlak syndrome (HPS), an autosomal recessive disorder that is characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This antibody (clone ME491) is shown to react with human primary and to some extent with metastatic melanoma tissues. (Ref.: Lai, X., et al. (2017). Oncol. Let. 13(6); 4245-4251; Tominaga, N., et al. (2014). Mol. Cancer 13; 134; Smith, M., et al. (1997). Melanoma Res. 7 (Suppl. 2), 163-170).

特异性

Clone ME491 specifically detects CD63 (LAMP-3) in human cells.

免疫原

Clear supernatant from SK-Mel-23 cell lysate.

应用

Anti-CD63 (LAMP3), clone ME491, Cat. No. MABF2159, is a mouse monoclonal antibody that detects CD63 antigen and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD63 (LAMP3) in human spleen and human bone marrow tissue sections.

Immunocytochemistry Analysis: A representative lot detected CD63 (LAMP3) in Immunocytochemistry applications (Atkinson, B., et. al. (1984). Cancer Res. 44(6):2577-81).

Flow Cytometry Analysis: A representative lot detected CD63 (LAMP3) in Flow Cytometry applications (Li, J., et. al. (2003). J Immunol. 171(6):2922-9).

Western Blotting Analysis: A representative lot detected CD63 (LAMP3) in Western Blotting applications (Smith, M., et. al. (1997). Melanoma Res. 7 Suppl 2:S163-70).

Immunohistochemistry Analysis: A representative lot detected CD63 (LAMP3) in Immunohistochemistry applications (Li, J., et. al. (2003). J Immunol. 171(6):2922-9).
Research Category
Inflammation & Immunology

质量

Evaluated by Western Blotting in human platelet lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected CD63 (LAMP3) in human platelet lysate.

目标描述

~43 kDa observed; 25.64 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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