Merck
所有图片(1)

MABF2774

Sigma-Aldrich

Anti-Dengue Virus 2 NS1 Antibody

登录to View Organizational & Contract Pricing

Select a Size

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

purified antibody

antibody product type

primary antibodies

克隆

164, monoclonal

分子量

calculated mol wt 48 kDa

species reactivity

virus

包装

antibody small pack of 100 μg

technique(s)

ELISA: suitable

同位素/亚型

IgG1κ

UniProt登记号

运输

dry ice

储存温度

2-8°C

target post-translational modification

unmodified

Gene Information

Unknown ... POLY(1494449)

比较类似商品

查看完整比较结果

显示差异

1 of 4

此商品
MABF2218MAB8701MAB8704
conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

-

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

ascites fluid

clone

164, monoclonal

clone

2H6, monoclonal

clone

15F3-1, monoclonal

clone

1H10-6, monoclonal

mol wt

calculated mol wt 48 kDa

mol wt

calculated mol wt 25.47 kDa, observed mol wt ~35 kDa

mol wt

-

mol wt

-

species reactivity

virus

species reactivity

influenza A virus

species reactivity

human

species reactivity

human

一般描述

Genome polyprotein (UniProt: P29990) following its synthesis is cleaved into 13 different proteins in Dengue virus 2. Dengue virus (DENV) is a mosquito-borne flavivirus and is one of the most common mosquito-transmitted viral infections. The DENV particle is about 500 nm in diameter and includes a positive-sense RNA genome with ~10,700 nucleotides and 3 structural proteins known as capsid (C), precursor membrane (prM), and envelope (E) proteins. In addition, the RNA genome of dengue virus also encodes seven nonstructural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5) that are shown to be essential for viral replication. DENV NS1, a 48-kDa glycoprotein (aa 776-1127 of genome polyprotein), is highly conserved among all flaviviruses and is essential for viral replication. It is expressed as a monomer in infected cells and after post-translational modification in the lumen of endoplasmic reticulum, it forms homodimers associated with organelle membranes and the cell membrane. NS1 is reported to be involved in immune evasion, pathogenesis, and viral replication. Once cleaved off the polyprotein, is targeted to three destinations: the viral replication cycle, the plasma membrane and the extracellular compartment. NS1 is secreted in a hexamer form, which is composed of three dimers with a detergent-sensitive hydrophobic central cavity and it plays a role against host immune response. NS1 is shown to induce expression of sialidases, heparanase, and activates cathepsin L, which activates heparinase via enzymatic cleavage. These effects are may contribute to the endothelial hyperpermeability commonly observed in severe dengue disease. During the recovery phase, NS1 is cleared from the circulation by antibody-mediated effects. (Ref.: Chen, RU et al. (2018). J. Biomed. Sci. 25(1); 58; Bosch, I., et al. (2017). Sci. Transl. Med. 9(409); eaan1589).

特异性

Clone 164 is a mouse monoclonal antibody that detects NS1 protein of Dengue virus 2. It targets an epitope within the N-terminal half.

免疫原

Recombinant Dengue Virus 2 NS1 protein.

应用

Quality Control Testing

Evaluated by ELISA with DENV2 NS1 recombinant protein.

ELISA Analysis: 1.0 μg/mL of this antibody detected recombinant Dengue Virus 2 NS1 protein.

Tested Applications

ELISA Analysis: A representative lot detected Dengue Virus 2 NS1 in ELISA applications (Bosch, I., et. al. (2020). PLoS Negl Trop Dis.

14(6):e0008203).Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
This mouse monoclonal Anti-Dengue Virus 2 NS1, clone 164, Cat. No. MABF2774, is a mouse monoclonal antibody that detects Dengue virus NS1 protein and is tested for use in ELISA.

外形

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at +2°C to +8°C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题?欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ (Lot或Batch)字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

  • 如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。

  • 如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。

未找到您寻找的产品?

部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。

索取COA

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门