Merck
所有图片(2)

文件

MABF3160

Sigma-Aldrich

Anti-N-Cadherin Antibody, clone 8C11

登录to View Organizational & Contract Pricing

Select a Size

Change View

Select a Size

Change View
别名:
Cadherin-2;CDw325;Neural Cadherin;CD325

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

purified antibody

antibody product type

primary antibodies

克隆

8C11, monoclonal

分子量

calculated mol wt 99.81 and 97.04 kDa
observed mol wt ~105 and 130 kDa

纯化方式

using protein G

species reactivity

hamster, human

包装

antibody small pack of 100 μg

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
inhibition assay: suitable
western blot: suitable

同位素/亚型

IgG1κ

表位序列

Extracellular domain

Protein ID登记号

UniProt登记号

运输

dry ice

target post-translational modification

unmodified

Gene Information

human ... CHD2(1000)

比较类似商品

查看完整比较结果

显示差异

1 of 4

此商品
C3865C1821C3678
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

whole antiserum

clone

8C11, monoclonal

clone

GC-4, monoclonal

clone

CH-19, monoclonal

clone

polyclonal

mol wt

calculated mol wt 99.81 and 97.04 kDa, observed mol wt ~105 and 130 kDa

mol wt

antigen 135 kDa

mol wt

antigen 135 kDa

mol wt

antigen 135 kDa

species reactivity

hamster, human

species reactivity

chicken, monkey, mouse, human, rabbit, rat

species reactivity

canine, frog, snake, guinea pig, chicken, pig, feline, bovine, rabbit, hamster, Psammomys (sand rat), goat, rat, human, sheep, mouse

species reactivity

rabbit, cat, chicken, human

Still not finding the right product?  

Give our Product Selector Tool a try.

一般描述

Cadherin-2 (UniProt: P19022; also known as CDw325, Neural cadherin, N-cadherin, CD325) is encoded by the CDH2 (also known as CDNH, NCAD) gene (Gene ID: 1000) in human. N-Cadherin is a single-pass type I membrane glycoprotein protein that plays a role in calcium-dependent cell adhesion. Its expression is observed in cells of the nervous tissue, vascular smooth muscle cells, and myocytes. N-cadherins preferentially interacts with themselves in a homophilic manner in connecting cells; may thus contribute to the sorting of heterogeneous cell types. N-cadherin are synthesized as precursor proteins with a signal peptide (aa 1-25) and a propeptide (aa 26-159) that are proteolytically cleaved to generate functional, mature protein. Newly synthesized proN-cadherin (aa 1-906) is phosphorylated and proteolytically processed prior to transport to the plasma membrane. The mature N-Cadherin contains an extracellular domain (aa 160-724) with five homologous repeats that mediates adhesion, a single pass transmembrane domain (aa 725-745), and a conserved cytoplasmic domain (aa 746-906) that interacts with catenins to link cadherins to the actin cytoskeleton. N-cadherin is well expressed in endothelial cells and plays an essential role in the maturation and stabilization of normal vessels. However, it also promotes tumor cell survival, migration and invasion, and a high level of its expression is often associated with poor prognosis. A 69 amino acid segment of EC-4 of N-cadherin is reported to be essential and sufficient to increase cell motility and promote epithelial to mesenchymal transition. (Ref.: Kim, JB., et al. (2000). J. Cell Biol. 151(6); 1193-1205; Navarro, P., et al. (1998). J. Cell Biol. 140(6); 1475-1484).

特异性

Clone 8C11 is a mouse monoclonal antibody that detects N-Cadherin. It targets an epitope within the extracellular domain.

免疫原

MBP-tagged recombinant fragment corresponding to 502 amino acids from the extracellular domain of human N-Cadherin.

应用

Quality Control Testing

Evaluated by Western Blotting in Human heart tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Cadherin-2 in Human heart tissue lysate.

Tested applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected N-Cadherin in Human brain and cerebellum tissue extracts.

Western Blotting Analysis: A representative lot detected N-Cadherin in Western Blotting applications (Kim, J.B., et al. (2000). J Cell Biol. 151(6):1193-1206).

Flow Cytometry Analysis: 1 μg from a representative lot detected N-Cadherin in one million KG-1 cells.

Inhibition Assay: A representative lot inhibited motility of BT20 breast cancer cells (Kim, J.B., et al. (2000). J Cell Biol. 151(6):1193-1206).

Flow Cytometry Analysis: A representative lot detected N-Cadherin in Flow Cytometry applications (Wahlig, S., et al. (2020). Sci Rep. 10(1):1681).

Immunocytochemistry Analysis: A representative lot detected Cadherin-2 in Immunocytochemistry applications (Navarro, P., et al. (1998). J Cell Biol.;140(6):1475-1484).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-N-Cadherin, clone 8C11, Cat. No. MABF3160, is a mouse monoclonal antibody that detects N-Cadherin and is tested for use in Flow Cytometry, Immunocytochemistry, Inhibition Assay, and Western Blotting.

外形

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

储存及稳定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题?欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ (Lot或Batch)字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

  • 如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。

  • 如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。

未找到您寻找的产品?

部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。

索取COA

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门