Klotho (UniProt: Q9UEF7; also known as EC: 184.108.40.206) is encoded by the KL gene (Gene ID:9365) in human. Klotho is a homodimeric, highly N-glycosylated, single-pass type I membrane protein that is present in kidney, placenta, small intestine, and prostate tissue. In kidney it is predominantly present in cortical renal tubules. A soluble secreted form (isoform 2, ~ 64 kDa) is present in serum and cerebrospinal fluid. The Klotho peptide generated by cleavage of the membrane-bound isoform (Isoform 1) is considered as an anti-aging circulating hormone that is reported to extend life span by inhibiting insulin/IGF1 signaling. It also acts as a cofactor for interaction of FGF23 with FGF R1, which negatively regulates the activity of 1-alpha-hydroxylase, the rate limiting enzyme in the synthesis of 1,25(OH)2D3. Klotho is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to generate the mature form that has an extracellular domain (aa 31-981), a short transmembrane domain (aa 982-1002), and a short cytoplasmic tail (aa 1003-1012). Deficiency of Klotho is reported to shorten life-span in animal models with a myriad of disorders associated with old age, such as arteriosclerosis, osteoporosis, and skin atrophy. Klotho deficient mice also display severe hyperphosphatemia and soft tissue calcification. On the contrary, over-expression of Klotho is shown to extend life-span in murine models by about 30% with side effects, such as mild insulin resistance and enhanced resistance to oxidative stress. Defects in KL gene has been linked to chronic renal failure complications.
Clone KL-234 is a rat monoclonal antibody that specifically detects Klotho. It targets an epitope with in 107 amino acids from the KL1 domain from the N-terminal region.
GST-tagged recombinant fragment corresponding to 107 amino acids from the extracellular domain from the N-terminal region of human Klotho.
Western Blotting Analysis: A 1:1,000 dilution from a represetative lot detected Klotho in 10 µg of mouse kidney tissue lysates.
Immunohistochemistry Analysis: A representative lot detected Klotho in Immunohistochemistry applications (Maltare, A., et. al. (2014). Monoclon Atib Immunodiagn Immunother. 33(6):420-7).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Klotho in Immunoprecipitation applications (Maltare, A., et. al. (2014). Monoclon Atib Immunodiagn Immunother. 33(6):420-7).
Western Blotting Analysis: A representative lot detected Klotho in Western Blotting applications (Maltare, A., et. al. (2014). Monoclon Atib Immunodiagn Immunother. 33(6):420-7).
Immunocytochemistry Analysis: A representative lot detected Klotho in Immunocytochemistry applications (Maltare, A., et. al. (2014). Monoclon Atib Immunodiagn Immunother. 33(6):420-7).
Anti-Klotho Antibody, clone KL-234, Cat. No. MABN1807, is a highly specific rat monoclonal antibody that targets Klotho and has been tested for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Evaluated by Western Blotting in human kidney tissue lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Klotho in 10 µg of human kidney tissue lysates.
~130 kDa observed; 116.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Rat monoclonal antibody as ascites with 0.1% sodium azide with 50% glycerol.
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
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