Merck
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MABN20

Sigma-Aldrich

Anti-HCN1 Antibody, clone N70/28

clone N70/28, from mouse

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别名:
hyperpolarization activated cyclic nucleotide-gated potassium channel 1, brain cyclic nucleotide gated channel 1, Brain cyclic nucleotide-gated channel 1
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

N70/28, monoclonal

species reactivity

rat

technique(s)

immunohistochemistry: suitable
western blot: suitable

同位素/亚型

IgG1κ

NCBI登记号

UniProt登记号

运输

wet ice

target post-translational modification

unmodified

Gene Information

human ... HCN1(348980)

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此商品
AB5884SAB5200024SAB5200035
Anti-HCN1 Antibody Chemicon®, from rabbit

Sigma-Aldrich

AB5884

Anti-HCN1 Antibody

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

N70/28, monoclonal

clone

polyclonal

clone

S70-28, monoclonal

clone

S114-10, monoclonal

species reactivity

rat

species reactivity

rat

species reactivity

mouse, rat, human

species reactivity

rat, human, mouse

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG1κ

isotype

-

isotype

IgG1

isotype

IgG1

一般描述

HCN1 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 proteins play an important role for motor learning and synapse formation through integration of cerebellar Purkinje cells. The two proteins also shape autonomous activity of single neurons and the periodicity of network oscillations. HCN1 expression is particularly high in the cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. Hyperpolarization of activated cation channels of the HCN gene family such as HCN1, contributes to spontaneous rhythmic activity in both heart and brain. HCN channels pass both Na+ and K+ with relative permeability ratio of ~0.15 to 0.25. The primary sequence of HCN pore region is clearly related to that of highly selective K+ channels. In contrast to nonselective CNG or Na+-channels, both K+ and HCN channels contain a GYG motif in the pore.

特异性

This antibody recognizes HCN1 at the cytoplasmic domain.

免疫原

Epitope: Cytoplasmic domain
Recombinant protein corresponding to rat HCN1 at the cytoplasmic domain.

应用

Detect HCN1 using this Anti-HCN1 Antibody, clone N70/28 validated for use in WB, IH.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected HCN1 in rat cerebellum and rat brain/inferior colliculus tissue.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

质量

Evaluated by Western Blot in rat brain lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected HCN1 on 10 µg of rat brain lysate.

目标描述

~120 kDa observed; consistent with a glycosylation of a 100kDa protein.

外形

Protein G
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Rat brain lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable


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Subcellular localization and expression of bamboo mosaic virus satellite RNA-encoded protein.
Palani, PV; Chiu, M; Chen, W; Wang, CC; Lin, CC; Hsu, CC; Cheng, CP; Chen, CM; Hsu, YH; Lin, NS
The Journal of General Virology null
Anikó Rátkai et al.
Scientific reports, 11(1), 3236-3236 (2021-02-07)
Homeostatic plasticity stabilizes neuronal networks by adjusting the responsiveness of neurons according to their global activity and the intensity of the synaptic inputs. We investigated the homeostatic regulation of hyperpolarization-activated cyclic nucleotide-gated (HCN) and T-type calcium (CaV3) channels in dissociated
Joon Ha Park et al.
International journal of molecular medicine, 44(5), 1801-1810 (2019-10-02)
Hyperpolarization‑activated cyclic nucleotide‑gated (HCN) channels have been known to participate in the regulation of neuronal excitability, synaptic transmission and long‑term potentiation in the hippocampus. The present study investigated transient ischemia‑induced changes of HCN1 and HCN2 expressions in the Cornu Ammonis

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