Retinoschisin (UniProt: O15537; also known as X-linked juvenile retinoschisis protein) is encoded by the RS1 (also known as XLRS1) gene (Gene ID: 6247) in human. Retinoschisin is discoidin domain-containing protein that is localized along the extracellular surfaces of rod and cone photoreceptors and bipolar cells including the synapses. It is undetectable in the inner plexiform layers and the inner nuclear layer. It is active in cell adhesion process during retinal development and maintains the cellular organization and synaptic structure of the retina. Retinoschisin is a homooctamer of 4 homodimers that are linked by disulfide bonds. It contains a single F5/8 type C domain. Deleterious mutations in RS1 encoding retinoschisin are associated with X-linked juvenile retinoschisis (RS), a common form of macular degeneration in males, which is characterized by a negative electroretinogram pattern and by a splitting of the inner retina. Approximately half of cases of X-linked retinoschisis have bilateral peripheral retinoschisis in the inferotemporal part of the retina. Retinoschisin levels are shown to be up-regulated during the differentiation of a retinoblastoma cell line.
Clone 3R10 detects Retinoschisin in murine retinal cells. It targets an epitope within 18 amino acids from the N-terminal region.
GST-tagged fusion protein containing a the LSSTEDEGEDPWYQKAC peptide fragment corresponding to amino acids 22-39 from the N-terminal region of human Retinoschisin precusor protein.
Immunofluorescence Analysis: A representative lot detected Retinoschisin in Immunofluorescence applications (Min, S.H., et. al. (2005). Mol Ther. 12(4):644-51; Weber, B.H., et. al. (2002). Proc Natl Acad Sci USA. 99(9):6222-7).
Western Blotting Analysis: A representative lot detected Retinoschisin in Western Blotting applications (Min, S.H., et. al. (2005). Mol Ther. 12(4):644-51; Wu, W.W., et. al. (2003). J Biol Chem. 278(30):28139-46).
Anti-Retinoschisin, clone 3R10, Cat. No. MABN2434, is a highly specific mouse monoclonal antibody that targets Retinoschisin and has been tested in Immunofluorescence and Western Blotting..
Evaluated by Western Blotting in mouse retinal tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Retinoschisin in 10 µg of mouse retinal tissue lysate.
~23 kDa observed; 25.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Protein G purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
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