Transferrin receptor protein 2 (UniProt: Q9UP52; also known as TfR2) is encoded by the TFR2 gene (Gene ID: 70360 in human. TfR2 is a single-pass type II membrane glycoprotein that plays a critical role in iron metabolism. It has a large C-terminal ectodomain and a small N-terminal cytoplasmic domain and shares 45% amino acid sequence with extracellular region of transferrin receptor (TfR). This homodimeric membrane receptor binds 2 molecules of transferrin and is internalized into endosomes that are acidified, resulting in the release of iron from transferrin. TfR2 contains a cytoplasmic internalization motif similar to TfR, and has 2 cysteines, which form inter subunit disulfide bonds, in the ectodomain proximal to the transmembrane domain. TfR2 mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. Both TfR and TfR2 bind diferric transferrin better than apo-transferrin at neutral pH. However, TFR2 displays about 25-fold lower affinity for transferrin compared to TfR and its expression is not controlled by cellular levels of iron. TfR2 is also suggested to be involved in involved in hepatocyte function and erythrocyte differentiation. Mutations in TFR2 gene are known to cause hemochromatosis 3 that is characterized by an iron overload where excess iron is deposited in several organs leading to organ failure. (Ref.: Kawabata H., et al (1999). J. Biol. Chem. 274 (30): 20826-20832; Vogt, TM et al. (2003). Blood 101(5):2008-2014.
Clone 9F8-1C11 sprcifically detects human TfR2 and does exhibit reactivity with TfR.
Purified ectodomains of the human TfR2.
Epitope: extracellular domain
Anti-TfR2, clone 9F8-1C11 Antibody, Cat. No. MABS1981, is a highly specific mouse monoclonal antibody, that targets Tfr2 and has been tested in Western Blotting, Immunoprecipitation, and Immunocytochemistry.
Immunocytochemistry Analysis: A representative lot detected Tfr2 in Immunocytochmeistry applications (Vogt, T.M., et. al. (2003). Blood. 101(5):2008-14; Zhao, N., et. al. (2013). Biochemistry. 52(19):3310-9).
Immunohistochemistry Analysis: A representative lot detected Tfr2 in Immunohistochemistry applications (Vogt, T.M., et. al. (2003). Blood. 101(5):2008-14).
Western Blotting Analysis: A representative lot detected Tfr2 in Western Blotting applications (Vogt, T.M., et. al. (2003). Blood. 101(5):2008-14; Byrne, S.L., et. al. (2013). PLoS One. 8(7):e70199; Zhao, N., et. al. (2013). Biochemistry. 52(19):3310-9).
Immunoprecipitation Analysis: A representative lot detected Tfr2 in Immunoprecipitation applications (Vogt, T.M., et. al. (2003). Blood. 101(5):2008-14; Johnson, M.B., et. al. (2004). Blood. 104(13):4287-93).
Epigenetics & Nuclear Function
Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Tfr2 in 10 µg of K562 cell lysaste.
~108 kDa observed; 88.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
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