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MABS1982

Sigma-Aldrich

Anti-TfR1 Antibody, clone 3B82A1

clone 3B82A1, from mouse

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别名:
Transferrin receptor protein 1, TR:TfR1, Trfr, T9, p90, Cd71
eCl@ss:
32160702

生物来源

mouse

质量水平

抗体形式

purified antibody

antibody product type

primary antibodies

克隆

3B82A1, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同位素/亚型

IgG1κ

NCBI登记号

UniProt登记号

运输

ambient

target post-translational modification

unmodified

Gene Information

human ... TFRC(7037)

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此商品
HPA028598SAB4700518SAB4200398
Anti-TFRC antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

HPA028598

抗-TFRC 兔抗

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

clone

3B82A1, monoclonal

clone

polyclonal

clone

MEM-75, monoclonal

clone

polyclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

mouse, rat, human

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:500-1:1000

technique(s)

flow cytometry: suitable

technique(s)

indirect immunofluorescence: 10-20 μg/mL using rat NRK cells, western blot: 2.5-5.0 μg/mL using whole extracts of human U-2-OS and mouse Neuro-2a cells

isotype

IgG1κ

isotype

-

isotype

IgG1

isotype

-

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一般描述

Transferrin receptor protein 1 (UniProt: P02786; also known as TR, TfR, TfR1, Trfr, T9, p90, CD71) is encoded by the TFRC gene (Gene ID: 7037) in human. Tfr is a disulfide-bonded homodimeric type II transmembrane glycoprotein that is involved in iron uptake. TfR is more abundantly expressed in rapidly dividing cells. Higher expression levels of TfR are seen in the placental trophoblast and hemoglobin-synthesizing reticulocytes, but expression is lost in mature erythrocytes. Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. Both TfR and TfR2 bind diferric transferrin better than apo-transferrin at neutral pH. However, TFR2 displays about 25-fold lower affinity for transferrin compared to TfR. TfR levels are regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3′-UTR. Up-regulated upon mitogenic stimulation. TfR can be N- and O-glycosylated, phosphorylated and palmitoylated, however, the serum form is only glycosylated. TfR can be proteolytically cleaved on Arg100 to produce the soluble serum form (sTfR). Mutations in TFRC gene result in immunodeficiency 46, an autosomal recessive disorder that is characterized by early-onset chronic diarrhea, recurrent infections, hypo- or agammaglobulinemia, intermittent neutropenia, and thrombocytopenia.

特异性

Clone 3B82A1 specifically detects transferrin receptor protein 1. It targets an epitope in the extracellullar domain.

免疫原

Epitope: extracellular domain
His-tagged recombinant ectodomain of human Transferrin receptor protein 1.

应用

Detects Transferrin receptor protein 1 using this mouse monoclonal Anti-TfR1, clone 3B82A1, Cat. No. MABS1982 and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Research Category
Signaling
Western Blotting Analysis: A representative lot detected TfR1 in Western Blotting applications (Chloupkova, M., et. al. (2010). Blood Cells Mol Dis. 44(1):28-33; Johnson, M.B., et. al. (2004). Blood. 104(13):4287-93; Chen, J., et. al. (2015). J Biol Chem. 290(12):7841-50; Vogt, T.M., et. al. (2003). Blood. 101(5):2008-14).

Immunocytochemistry Analysis: A representative lot detected TfR1 in Immunocytochemistry applications (Johnson, M.B., et. al. (2007). Mol Cell Biol. 18(3):743-54; Vogt, T.M., et. al. (2003). Blood. 101(5):2008-14).

Immunoprecipitation Analysis: A representative lot detected TfR1 in Immunoprecipitation applications (Johnson, M.B., et. al. (2004). Blood. 104(13):4287-93; Vogt, T.M., et. al. (2003). Blood. 101(5):2008-14).

质量

Evaluated by Flow Cytometry in K562 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected TfR1 in 1X10E6 K562 cells.

目标描述

84.87 kDa calculated.

外形

Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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