MABT841M

Sigma-Aldrich

Anti-Podoplanin Antibody, clone LpMab-12

clone LpMab-12, from mouse

别名:
Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A
eCl@ss:
32160702
NACRES:
NA.41
价格与库存信息目前不能提供

Quality Level

biological source

mouse

antibody product type

primary antibodies

clone

LpMab-12, monoclonal

species reactivity

human, monkey

packaging

antibody small pack of 25 μg

application(s)

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable (paraffin)

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

Gene Information

human ... PDPN(10630)

General description

Podoplanin (UniProt: Q86YL7; also known as Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A) is encoded by the PDPN (also known as GP36) gene (Gene ID: 10630) in human. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. Six isoforms of podoplanin have been described that are produced by alternative splicing. It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form. Podoplanin contains an extracellular domain (aa 23-131), a helical domain (aa 132-152) and a cytoplasmic region (aa 153-162). Podoplanin possesses three tandem repeat of platelet aggregation-stimulating (PLAG) domains in its N-terminus. Among the PLAG domains, sialylated O-glycan on Thr52 of PLAG3 is essential for the binding to C-type lectin-like receptor-2 (CLEC-2). Interaction of human podoplanin with CLEC-2 mainly involves Glu47 and Asp48 in the platelet PLAG3. Clone LpMab-12 specifically detects sialylated O-Glycan on Thr52 of platelet aggregation-stimulating domain of human podoplanin. It also reacts with the synthetic glycopeptide of hPDPN, corresponding to 38 54 amino acids that carries 2-6 sialylated N-acetyl-D-galactosamine (GalNAc) on Thr52. The minimal epitope of LpMab-12 is identified as Asp49 Pro53 of hPDPN. (Ref.: Kato, Y et al. (2016). PLoS ONE 11(3): e0152912).

Specificity

Clone LpMab-12 detects Podoplanin in human and monkey cells. It targets an epitope within the N-terminal half. It specifically detects Sialylated O-Glycan on Thr52 of platelet aggregation-stimulating domain of human podoplanin. The minimal epitope of LpMab-12 is identified as Asp49-Pro53.

Immunogen

LN229 glioma cells expressing human Podoplanin.

Application

Anti-Podoplanin, clone LpMab-12, Cat. No. MABT841, is a mouse monoclonal antibody that detects human and monkey podoplanin and has been tested for use in ELISA, Flow Cytometry, and Immunohistochemistry (Paraffin).
Research Category
Cell Structure
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Podoplanin in human testis cancer tissue.

ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).

Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).

Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).

Quality

Evaluated by Immunohistochemistry in human colon cancer tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Podoplanin in human colon cancer tissue sections.

Target description

16.70 kda calculated.

Physical form

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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