Merck
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OP29

Sigma-Aldrich

p53(Ab-3)(突变型)小鼠单克隆抗体(PAb240)

liquid, clone PAb240, Calbiochem®

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生物来源

mouse

质量水平

抗体形式

purified antibody

antibody product type

primary antibodies

克隆

PAb240, monoclonal

形式

liquid

包含

≤0.1% sodium azide as preservative

species reactivity

mouse, rat, human, chicken, hamster, bovine

should not react with

Xenopus

manufacturer/tradename

Calbiochem®

储存条件

do not freeze

同位素/亚型

IgG1

运输

wet ice

储存温度

2-8°C

target post-translational modification

unmodified

Gene Information

human ... TP53(7157)

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antibody form

purified antibody

clone

PAb240, monoclonal

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DO-7, monoclonal

clone

PAb421, monoclonal

clone

PAb1620, monoclonal

form

liquid

form

liquid

form

liquid

form

liquid

species reactivity

mouse, rat, human, chicken, hamster, bovine

species reactivity

bovine, monkey, human

species reactivity

rabbit, monkey, human, mouse, rat

species reactivity

human, mouse, rat, primate, bovine

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

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一般描述

在非变性条件下,通过免疫沉淀、免疫荧光和流式细胞术识别~53 kDa突变型p53蛋白。在变性条件下通过免疫印迹和石蜡切片识别突变型和野生型p53。
该抗p53(Ab-3)(突变体)小鼠mAb(PAb240)经验证可用于FC、冰冻切片、凝胶阻滞、免疫印迹、IF、IP和石蜡切片,以检测p53(Ab-3)(突变体)。
通过用指定的免疫原免疫BALB/c小鼠并将脾细胞与SP2小鼠骨髓瘤细胞融合而产生的纯化小鼠单克隆抗体(请参阅应用参考)。在非变性条件下,识别~53 kDa突变型p53蛋白。在变性条件下识别突变型和野生型p53蛋白。

免疫原

由p53的氨基酸14-389与β-半乳糖苷酶融合而成的重组蛋白
表位:氨基酸213-217内

应用

流式细胞仪(1-20 µg/ml)

冷冻切片(10 µg/ml)

凝胶阻滞(请参阅备注)

免疫印迹(5 µg/ml)

免疫荧光(1-20 µg/ml,请参阅应用参考)

免疫沉淀(每个样品1 µg)

石蜡部分(请参阅应用参考)

包装

请参考特定浓度批号的标签。

警告

毒性:标准处理(A)

外形

溶于50 mM磷酸钠缓冲液(pH 7.5),含0.2%明胶。

分析说明

阳性对照
A431、Hs27(野生型p53)或SK-BR-3细胞或乳腺癌组织
阴性对照
SK-OV-3细胞

其他说明

El-Deiry, W.S., et al. 1994.Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994.Cancer Res.54, 4855.
Barak, Y., et al. 1993.EMBO J.12, 461.
Kastan, M.B., et al. 1992.Cell71, 587.
Kuerbitz, S.J.1992.Proc.Natl.Acad.Sci. USA89, 7491.
Lane, D.P.1992.Nature358, 15.
Kastan, M.B., et al. 1991.Cancer Res.51, 6304.
在非变性条件下(免疫沉淀、免疫荧光和冰冻切片),抗p53(Ab-3)不识别正常(野生型)p53蛋白;它识别通过激活突变或变性暴露的表位。在变性方案(免疫印迹和石蜡切片)中,抗p53(Ab-3)将识别突变型和野生型p53。不会识别某些具有RHSVV表位突变的p53分子,但会与具有RHSVV表位的TFIIIA反应。对于凝胶阻滞分析,使用目录编号OP29L,复溶于100 µl缓冲液中。在单个系统中,应对抗体进行滴定以获得最佳结果。

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Koji Nishio et al.
Histochemistry and cell biology, 123(3), 263-273 (2005-03-03)
The cytoskeleton of senescent cells was systematically studied using senescent and young fibroblasts. In the cell senescence, skin fibroblasts extraordinarily produced vimentin in contrast to actin and tubulin, which were down-regulated. Among the focal adhesion proteins, paxillin and c-Src decreased
Chinthalapally V Rao et al.
Neoplasia (New York, N.Y.), 15(9), 1018-1027 (2013-09-13)
Lung cancer is the leading cause of cancer deaths worldwide. Expression of the p53 tumor suppressor protein is frequently altered in tobacco-associated lung cancers. We studied chemopreventive effects of p53-modulating agents, namely, CP-31398 and Prima-1, on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced lung adenoma
Maira M Pires et al.
Cancer biology & therapy, 14(3), 246-253 (2013-01-08)
Breast cancer can be classified into different molecular subtypes with varying clinical and pathological characteristics. The basal-like breast cancer subtype represents one of the most aggressive and lethal types of breast cancer, and due to poor mechanistic understanding, it lacks
Monika Aggarwal
Cancers, 15(3) (2023-02-12)
We previously reported that phenethyl isothiocyanate (PEITC), a dietary-related compound, can rescue mutant p53. A structure-activity relationships study showed that the synthetic analog 2,2-diphenylethyl isothiocyanate (DPEITC) is a more potent inducer of apoptosis than natural or synthetic ITCs. Here, we
Preethi H Gunaratne et al.
Cancer, 125(14), 2409-2422 (2019-04-24)
Over 96% of high-grade ovarian carcinomas and 50% of all cancers are characterized by alterations in the p53 gene. Therapeutic strategies to restore and/or reactivate the p53 pathway have been challenging. By contrast, p63, which shares many of the downstream

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