Merck
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PC635

Sigma-Aldrich

PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb

liquid, Calbiochem®

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别名:
Anti-Nibrin (pSer³⁴³) Rabbit pAb

生物来源

rabbit

质量水平

抗体形式

diluted serum

antibody product type

primary antibodies

克隆

polyclonal

形式

liquid

不包含

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

同位素/亚型

IgG

运输

wet ice

储存温度

−20°C

target post-translational modification

phosphorylation (pSer343)

Gene Information

human ... NBN(4683)

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AP1063AP1064PC386
Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

antibody form

diluted serum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

form

liquid

form

liquid

form

liquid

form

liquid

species reactivity

human

species reactivity

mouse, human, rat

species reactivity

human

species reactivity

rat, mouse, human

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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一般描述

Rabbit polyclonal antibody supplied as adsorbed diluted serum. Recognizes the ~95 kDa nibrin (NBS1) protein phosphorylated at Ser343.
Recognizes the ~95 kDa nibrin protein phosphorylated at Ser343 in δ-irradiated lymphoblastoid cells.
This PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of NBS1 (pSer³⁴³).

免疫原

Human
a synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser³⁴³ phosphorylation site of human nibrin, conjugated to diphtheria toxoid

应用

ELISA (1:16,000)

Immunoblotting (1:1000)

Immunoprecipitation (1 µl/500 µg cell extract)

警告

Toxicity: Standard Handling (A)

外形

Serum diluted in PBS.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
γ-irradiated lymphoblastoid cells

其他说明

Gatei, M., et al. 2000. Nat. Genet.25, 115.
Lim, D.S., et al. 2000. Nature404, 613.
Wu, G., et al. 2000. J. Biol. Chem.275, 30618.
Zhong, Q., et al. 1999. Science285, 747.
Carney, J.P., et al. 1998. Cell93, 477.
Trujillo, K.M., et al. 1998. J. Biol. Chem.273, 21447.
Varon, R., et al. 1998. Cell93, 467.
This antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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