SCC049

O9-1 Mouse Cranial Neural Crest Cell Line

Mouse

• 别名: Multipotent mesenchymal cells
• 分類程式碼代碼: 41106514
• eCl@ss: 32011203
• NACRES: NA.81

属性

• Product Name: O9-1 Mouse Cranial Neural Crest Cell Line, stably expresses stem cell markers and neural crest markers
• 生物源: mouse
• 品質等級: 100
• 技術: cell culture | stem cell: suitable
• 運輸包裝: liquid nitrogen

说明

一般說明

Cranial neural crest cells give rise to ectomesenchymal derivatives such as cranial bones, cartilage, smooth muscle, dentin, as well as melanocytes, corneal endothelial cells, and neurons and glial cells of the peripheral nervous system. Previous studies have suggested that although multipotent stem-like cells may exist during the course of cranial neural crest development, they are transient, undergoing lineage restriction early in embryonic development.

Whole-genome expression profiling of O9-1 cells revealed that this line stably expresses stem cell markers (CD44, Sca-1, and Bmi1) and neural crest markers (AP-2a, Twist1, Sox9, Myc, Ets1, Dlx1, Dlx2, Crabp1, Epha2, and Itgb1). O9-1 cells are capable of contributing to cranial mesenchymal (osteoblast and smooth muscle) neural crest fates when injected into E13.5 mouse cranial tissue explants and chicken embryos. These results suggest that O9-1 cells represent multipotent mesenchymal cranial neural crest cells. The O9-1 cell line should serve as a useful tool for investigating the molecular properties of differentiating cranial neural crest cells. The O9-1 cell line can be propagated and passaged for at least 10 passages, and can differentiate into osteoblasts, chondrocytes, smooth muscle cells, and glial cells (Ishii, 2012).

Ishii, M., et al. (2012) A stable cranial neural crest cell line from mouse. Stem Cells Dev. 21(17): 3069-3080.

細胞系描述

Neural Lineage Cells

應用

Research Category
Neuroscience

Stem Cell Research

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact [email protected].

成分

1) ≥1X10⁶ viable O9-1 Mouse Cranial Neural Crest Cell Line: (Catalog No. SCC049). Derived from mass cultures of Wnt1-Cre; R26R-GFP reporter-expressing cranial neuronal crest cells from E8.5 mouse embryos.

品質

• Each vial contains ≥ 1X10⁶ viable cells at passage 20 -22
• Cells tested negative for infectious disease by a murine PCR panel (Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services)
• Cells tested negative for mycoplasma contamination

外觀

Product is supplied frozen in 10% DMSO and 90% expansion medium.

儲存和穩定性

O9-1 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable