Merck
所有图片(2)

SCC066

Sigma-Aldrich

血脑屏障hCMEC/D3细胞系

The hCMEC/D3 BBB cell line has been extensively characterized for brain endothelial phenotype and is a model of human blood-brain barrier (BBB) function.

别名:
Human BBB cell line, Human blood-brain barrier cell line, Human cerebral endothelial cell line, CMEC/D3 cell Line, hCMEC/D3 cell line
eCl@ss:
32011203

生物来源

human

质量水平

technique(s)

cell culture | mammalian: suitable
drug transporter assay: suitable

运输

liquid nitrogen

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此商品
SCM003SCC134VIV402003
technique(s)

cell culture | mammalian: suitable, drug transporter assay: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell based assay: suitable

technique(s)

-

shipped in

liquid nitrogen

shipped in

dry ice

shipped in

ambient

shipped in

-

Quality Level

100

Quality Level

300

Quality Level

100

Quality Level

-

一般描述

HCMEC/D3细胞系源自人颞叶微血管,该微血管是从手术中切除的组织中分离出来的,以控制癫痫。主要分离物富含脑内皮细胞(CEC)。在第一代中,通过用人端粒酶(hTERT)和SV40大T抗原的催化亚基进行慢病毒载体转导,依次使细胞永生化,然后通过有限稀释克隆选择性分离CEC,并广泛表征克隆的脑内皮表型。这种脑微血管内皮细胞系代表了一种这样的人血脑屏障模型,该模型易于生长,并且可以进行与中枢神经系统(CNS)相关的病理学和药物转运机制的细胞和分子研究。

参考文献:
Couraud PO, et al. (2008) The human brain endothelial cell line hCMEC/D3 as a human blood‐brain barrier model for drug transport studies.Fluids Barriers CNS.2013 Mar 26;10(1):16.
血–脑屏障(BBB)是一种高度选择性的通透性屏障,可将循环血液与中枢神经系统中的脑细胞外液分开。血–脑屏障是由毛细血管内皮细胞形成的,毛细血管内皮细胞通过具有极高电阻率的紧密连接连接。血–脑屏障允许水、某些气体和脂溶性分子通过被动扩散,以及对神经功能至关重要的分子(例如葡萄糖和氨基酸)的选择性运输。另一方面,血–脑屏障可以通过P-糖蛋白介导的主动转运机制阻止亲脂性潜在神经毒素的进入。星形胶质细胞是形成血–脑屏障所必需的。血–脑屏障(BBB)可防止大多数药物从血液进入大脑。血脑屏障的存在使得开发新的脑部疾病治疗方法或用于脑部神经成像的新放射性药物变得困难。

细胞系描述

内皮细胞

应用

HCMEC/D3 BBB细胞系已被广泛表征为脑内皮表型,并且是人血脑屏障(BBB)功能的模型。
根据产品文件中详述的“限制使用协议”条款,本产品预期用于销售和仅用于内部非商业研究用途。有关任何其他用途的信息,请联系licensing@emdmillipore.com。
研究子类别
神经退行性疾病
研究类别
神经科学

质量

• 每瓶含有≥ 1X10^6个活细胞。
•通过PCR检测细胞,对甲型、乙型、丙型肝炎、HPV、疱疹和HIV-1 & 2型病毒呈阴性。
•细胞对支原体污染呈阴性。

储存及稳定性

血脑屏障HCMEC/D3细胞应储存在液氮中。细胞可以培养至少10代,而不会显著影响细胞标志物的表达和功能。

免责声明

除非在产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得对人或动物用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用。

本产品含有转基因生物(GMO)。在欧盟范围内,转基因物种分别受欧洲议会和理事会的2001/18 /EC和2009/41/EC指令及其成员国中国家所实施规范的约束。这项法律规定{HCompany}有义务要求您提供某些信息,以及有关处理 GMO 的机构的信息。请点击这里,获得最终用户声明(EUD)表格

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable


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