SCC100

MCF-7/S0.5 Human Breast Cancer Cell Line

MCF-7/S0.5 is a tamoxifen-sensitive cell line established from the MCF-7 human breast cancer cell line by adaption to growth in low serum conditions.

• 别名: MCF7 S0.5 ; ; Human breast cancer cell line
• eCl@ss: 32011203

属性

• 生物来源: human
• 质量水平: 100
• technique(s): cell culture | mammalian: suitable
• 运输: ambient

说明

一般描述

Estrogen receptor-positive breast cancer is the most common type of breast cancer. About 80% of all breast cancers express the estrogen receptor (ER+) and depend on estrogen to support the growth and spread of the cancer cells. Hormonal therapy, such as tamoxifen, may help slow or stop the growth of ER+ breast cancers by blocking the actions of estrogen. Primary or acquired tamoxifen resistance poses a major clinical challenge in the treatment of ER+ breast cancer. The MCF-7/S0.5 is the parental line from which many tamoxifen-resistant cell lines have been derived and is utilized as a parental control to its derivative cell lines in parallel experiments.

The presence of hormones in the serum required in tissue culture media complicates the demonstration of specific hormone and antihormone effects. The MCF-7/S0.5 human breast cancer cells have been adapted to long-term growth in low serum concentration (0.5% fetal bovine serum) by a stepwise reduction of the medium serum content from 5% to 0.5% FBS. MCF-7/S0.5 cells express both estrogen and progesterone receptors and are sensitive to tamoxifen.

细胞系描述

Cancer Cells

应用

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

质量

• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.

储存及稳定性

MCF-7/S0.5 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 1
• 闪点(F): 188.6 °F - (Dimethylsulfoxide)
• 闪点(C): 87 °C - (Dimethylsulfoxide)