Merck
所有图片(2)

文件

SCC109

Sigma-Aldrich

AC16 人心肌细胞细胞系

AC16 Human Cardiomyocytes can be serially passaged and can differentiate when cultured in mitogen-free medium. The cells may be used to study developmental regulation of cardiomyocytes.

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别名:
AC16 Ventricular Cardiomyocyte Cell Line
eCl@ss:
32011203

生物来源

human

质量水平

technique(s)

cell culture | mammalian: suitable

运输

dry ice

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SCC065SCC043SCC067
HL-1 Cardiac Muscle Cell Line HL-1 Cardiac Muscle Cell Line has been extensively characterized and is a valuable model system to address questions of cardiac biology at the cellular & molecular levels.

Sigma-Aldrich

SCC065

HL-1 心肌细胞系

C28/I2 Human Chondrocyte Cell Line C28/I2 Human Chondrocyte Cell Line is widely used as a model cell line for studying normal and pathological cartilage repair mechanisms related to chondrocyte biology and physiology.

Sigma-Aldrich

SCC043

C28/I2 人软骨细胞系

HMC-1.1 Human Mast Cell Line HMC-1.1 human mast cell line is a variant subline of the HMC-1 cell line and possesses the V560G KIT mutation.

Sigma-Aldrich

SCC067

HMC-1.1 人肥大细胞系

technique(s)

cell culture | mammalian: suitable

technique(s)

cell based assay: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

shipped in

-

shipped in

ambient

shipped in

dry ice

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

一般描述

AC16人心肌细胞系是一种增殖细胞系,源自成人心室心脏组织的原代细胞与SV40转化的尿苷营养缺陷型人成纤维细胞融合,不含线粒体DNA。 在无尿苷培养基中筛选去除未融合的成纤维细胞后,将融合细胞进一步亚克隆,然后筛选是否存在SV40大T-ag,β-肌球蛋白重链(βMHC)和连接蛋白43(CX-43)

通过改变培养条件和沉默SV40 T-ag的表达可以控制AC16细胞的增殖和分化。 这些细胞可用于在正常发育过程中,并且在细胞、细胞器和分子水平的病理条件下研究心脏基因的表达和功能。

细胞系描述

心肌细胞

应用

研究类别
心脏

心血管疾病

毒性
该产品可出售给学术机构或非营利性实体以供研究使用。有关商业许可的信息,请联系licensing@emdmillipore.com。

质量

• 每小瓶含有≥ 1X106个活细胞。
•通过PCR检测细胞,对HPV-16、HPV-18、甲型、乙型、丙型肝炎和HIV-1 & 2型病毒呈阴性。
•细胞对支原体污染呈阴性。
•通过STR分析对每批细胞进行基因分型,以验证细胞系的唯一身份。

储存及稳定性

AC16人心肌细胞株应保存在液氮中。在初始解冻后,细胞可以培养至少10代,而不会显著影响细胞标志物的表达和功能。

免责声明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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The cellular mechanisms involved in myocardial ischemia/reperfusion injury (I/R) pathogenesis are complex but attributable to reactive oxygen species (ROS) production. ROS produced by coronary endothelial cells, blood cells (e.g., leukocytes and platelets), and cardiac myocytes have the potential to damage
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P Litzkas et al.
Molecular and cellular biology, 4(11), 2549-2552 (1984-11-01)
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