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04942078001

Roche

分散酶®II (中性蛋白酶, II级)

lyophilized, from bacterial, Roche, pkg of 5 × 1 g

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别名:
解离酶, 组织解离, 中性蛋白酶
EC 号:
3.4.24.4 (BRENDA, IUBMB)

质量水平

100

生物来源

bacterial

无菌性

non-sterile

形式

lyophilized

specific activity

≥0.8 units/mg protein (37 °C, casein as substrate, pH 7.5)

包装

pkg of 5 × 1 g

应用

sample preparation: suitable

optimum pH

6.0-8.5

运输

wet ice

一般描述

分散酶是一种中性、快速反应、温和的蛋白酶，能将表皮从真皮中完整分离。它还能将培养的上皮细胞从基质中完整分离出来。其可分裂基底膜区，使上皮细胞保持完整。

特异性

分散酶是一种非特异性的金属蛋白酶。

应用

分散酶®用于从多种不同的组织和器官制备细胞。该酶已经证实是一种快速、有效但温和破坏组织胞外基质的制剂，可释放用于细胞培养的单细胞（原代细胞培养）或收集培养细胞以转至新底物中（继代培养）。此外，它还可用来防止细胞悬浮培养过程中形成不必要的团块。

特点和优势

  • Rapid, effective, yet gentle agent that liberates cells with minimal cell damage
  • Maintains cell membrane integrity
  • Non-mammalian source - free of mycoplasma and animal virus contamination
  • Extremely stable to influences of temperature, pH, and interference by serum components
  • Easily inactivated by chelating agents or by dilution
  • Delivers higher activity and convenience

单位定义

一单位是指在pH 7.5、+37 °C反应条件下，在1分钟内释放出对应1 μmol (181 μg)酪氨酸的Folin-阳性的氨基酸和肽类所需的酶量。
Roche分散酶®II单位与日本文献中引用的分散酶单位（其中分散酶®浓度为1，000到2，000单位/ml的情况不常见）的实际比较结果表明，Roche的1单位分散酶大致相当于日本的600单位。
Roche Applied Science的1单位®相当于181蛋白酶单位（PU），其中PU为在pH 7.5和+37 °C条件下，在1分钟内释放相当于1 μg酪氨酸的氨基酸所需的酶量。

制备说明

激活剂：最佳Ca2+ 浓度为2mM。酶制剂含有维持最佳活性所需的足量Ca2+。
抑制剂：EDTA、EGTA、Hg2+、其他重金属。分散酶不受血清抑制。
工作浓度：0.6至2.4 U/ml
工作溶液：储备溶液和工作溶液制备：

制备10mg/ml的储备溶液，将分散酶®II冻干粉溶于HEPES-缓冲盐溶液中（50 mM HEPES/KOH pH 7.4, 150 mM NaCl）。制备工作溶液，将上述储备溶液用分离细胞培养基稀释至终浓度为0.6到2.4U/ml。请注意，建议浓度不要高于2.4U/ml。为实现最佳结果，用0.22μm滤膜过滤工作溶液。
储存条件（工作溶液）：-15 至 -25 °C
复溶的储备溶液在2至8℃下可稳定保存2周。如需保存2个月以上，储备溶液需分装冻存。避免反复冻融! 采用PBS稀释的工作溶液在2至8℃下可稳定保存3天。

储存及稳定性

2至8 °C储存。（干燥储存！）

其他说明

仅用于生命科学研究。不可用于诊断。

法律信息

Dispase is a registered trademark of Godo Shusei Co., Ltd.

象形图

Exclamation markHealth hazard
GHS07,GHS08

警示用语：

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 1

闪点(F)

does not flash

闪点(C)

does not flash

分析证书

原产地证书 (CofO)

更多文件

Product Information Sheet - 4942078001
Enzyme Explorer
化学品安全说明书
Bulk Quote-Order Product
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