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04942078001

Roche

分散酶®II (中性蛋白酶, II级)

lyophilized, from bacterial, Roche, pkg of 5 × 1 g

别名:
解离酶, 组织解离, 中性蛋白酶

质量水平

100

生物来源

bacterial

无菌性

non-sterile

形式

lyophilized

specific activity

≥0.8 units/mg protein (37 °C, casein as substrate, pH 7.5)

包装

pkg of 5 × 1 g

应用

sample preparation: suitable

optimum pH

6.0-8.5

运输

wet ice

一般描述

分散酶是一种中性、快速反应、温和的蛋白酶,能将表皮从真皮中完整分离。它还能将培养的上皮细胞从基质中完整分离出来。其可分裂基底膜区,使上皮细胞保持完整。

特异性

分散酶是一种非特异性的金属蛋白酶。

应用

分散酶®用于从多种不同的组织和器官制备细胞。该酶已经证实是一种快速、有效但温和破坏组织胞外基质的制剂,可释放用于细胞培养的单细胞(原代细胞培养)或收集培养细胞以转至新底物中(继代培养)。此外,它还可用来防止细胞悬浮培养过程中形成不必要的团块。

特点和优势

  • Rapid, effective, yet gentle agent that liberates cells with minimal cell damage
  • Maintains cell membrane integrity
  • Non-mammalian source - free of mycoplasma and animal virus contamination
  • Extremely stable to influences of temperature, pH, and interference by serum components
  • Easily inactivated by chelating agents or by dilution
  • Delivers higher activity and convenience

单位定义

一单位是指在pH 7.5、+37 °C反应条件下,在1分钟内释放出对应1 μmol (181 μg)酪氨酸的Folin-阳性的氨基酸和肽类所需的酶量。
Roche分散酶®II单位与日本文献中引用的分散酶单位(其中分散酶®浓度为1,000到2,000单位/ml的情况不常见)的实际比较结果表明,Roche的1单位分散酶大致相当于日本的600单位。
Roche Applied Science的1单位®相当于181蛋白酶单位(PU),其中PU为在pH 7.5和+37 °C条件下,在1分钟内释放相当于1 μg酪氨酸的氨基酸所需的酶量。

制备说明

激活剂:最佳Ca2+ 浓度为2mM。酶制剂含有维持最佳活性所需的足量Ca2+
抑制剂:EDTA、EGTA、Hg2+、其他重金属。分散酶不受血清抑制。
工作浓度:0.6至2.4 U/ml
工作溶液:储备溶液和工作溶液制备:

制备10mg/ml的储备溶液,将分散酶®II冻干粉溶于HEPES-缓冲盐溶液中(50 mM HEPES/KOH pH 7.4, 150 mM NaCl)。制备工作溶液,将上述储备溶液用分离细胞培养基稀释至终浓度为0.6到2.4U/ml。请注意,建议浓度不要高于2.4U/ml。为实现最佳结果,用0.22μm滤膜过滤工作溶液。
储存条件(工作溶液):-15 至 -25 °C
复溶的储备溶液在2至8℃下可稳定保存2周。如需保存2个月以上,储备溶液需分装冻存。避免反复冻融! 采用PBS稀释的工作溶液在2至8℃下可稳定保存3天。

储存及稳定性

2至8 °C储存。(干燥储存!)

其他说明

仅用于生命科学研究。不可用于诊断。

法律信息

Dispase is a registered trademark of Godo Shusei Co., Ltd.

象形图

Exclamation markHealth hazard

警示用语:

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 1

闪点(F)

does not flash

闪点(C)

does not flash

分析证书

原产地证书 (CofO)

Zhonghou Wang et al.
ACS pharmacology & translational science, 3(4), 749-758 (2020-08-22)
As a result of the COVID-19 pandemic, evidence revealed that SARS-CoV-2 infection caused taste loss at a rate higher than that of influenza. ACE2, the entry receptor of SARS-CoV-2, has been identified in the oral epithelium; however, it is unclear...
Johanna Tukler Henriksson et al.
Investigative ophthalmology & visual science, 56(8), 4186-4197 (2015-07-02)
To investigate the effects of IL-13 on goblet cell proliferation, differentiation, and expression of mucin and immunomodulatory genes. Explants were excised from the conjunctiva of young C57BL/6 mice. Cultures received 200 μL per week of either Keratinocyte media (KSFM) or...
Chen Chen et al.
Bone research, 8, 7-7 (2020-03-05)
Lumbar degenerative disc diseases cause low back pain (LBP). The maintenance of the height and stability of the intervertebral disc (IVD) space is an effective treatment for LBP. The following study evaluated the effects of fibroblast injection on intervertebral disc...
Alice S Kaanta et al.
Breast cancer research : BCR, 15(4), R65-R65 (2013-08-21)
The mouse mammary gland provides a powerful model system for studying processes involved in epithelial tissue development. Although markers that enrich for mammary stem cells and progenitors have been identified, our understanding of the mammary developmental hierarchy remains incomplete. We...
Andrea J De Micheli et al.
Cell reports, 30(10), 3583-3595 (2020-03-12)
Muscle regeneration relies on the regulation of muscle stem cells (MuSCs) through paracrine signaling interactions. We analyzed muscle regeneration in mice using single-cell RNA sequencing (scRNA-seq) and generated over 34,000 single-cell transcriptomes spanning four time-points. We identified 15 distinct cell...

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