- 04942086001
04942086001
Roche
Dispase ® I（中性蛋白酶，I 级）
lyophilized, ≥10 units/mg protein (20 U/vial (37 °C, casein as substrate, pH 7.5)), optimum pH 6.0-8.5
Recommended Products
形式
lyophilized
质量水平
specific activity
≥10 units/mg protein (20 U/vial (37 °C, casein as substrate, pH 7.5))
包装
pkg of 10 × ~2 mg
manufacturer/tradename
Roche
最佳pH
6.0-8.5
运输
wet ice
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This Item | P5985 | 04942078001 | SAE0110 |
|---|---|---|---|
| form lyophilized | form liquid | form lyophilized | form aqueous solution |
| specific activity ≥10 units/mg protein (20 U/vial (37 °C, casein as substrate, pH 7.5)) | specific activity ≥16 U/g | specific activity ≥0.8 units/mg protein (37 °C, casein as substrate, pH 7.5) | specific activity - |
| manufacturer/tradename Roche | manufacturer/tradename - | manufacturer/tradename Roche | manufacturer/tradename - |
| optimum pH 6.0-8.5 | optimum pH - | optimum pH 6.0-8.5 | optimum pH - |
| shipped in wet ice | shipped in - | shipped in - | shipped in dry ice |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level - |
一般描述
特异性
应用
特点和优势
- 快速、有效、温和的试剂，可释放完整细胞
- 维持细胞膜完整性
- 非哺乳动物来源-无支原体和动物病毒污染
- 对温度、pH 值和血清成分干扰的影响非常稳定
- 易被螯合剂或稀释液灭活
- 提供更高的活性和便利性
内容
10 瓶，每瓶约 2 mg（= 20U/瓶）。
产品规格
抑制剂： EDTA，EGTA，Hg 2 + ，其他重金属。分散酶不被血清抑制。
单位定义
分散酶®的 RAS 单位与日本文献中引用的 RAS 单位（1,000 至 2,000 分散酶®单位/ml 的浓度并不少见）的实际比较表明，1 个罗氏分散酶® I 应用科学单位约等于 416 个日本分散酶单位®。
1 个单位的罗氏应用分散酶®等于 181 个蛋白酶单位 (PU)，以 pH 7.5 和 + 37°C 下相当于每分钟每毫升 1 μg 酪氨酸的氨基酸释放量测量。
外形
制备说明
工作浓度： 0.6～2.4 U/mL
工作溶液：贮备液和工作溶液的制备：
为制备 10 mg/mL 贮备液，将冻干分散酶® I 酶溶于 HEPES 缓冲盐水 (50 mM HEPES/KOH pH 7.4，150 mM NaCl) 中。为制备工作液，用分离细胞的培养基稀释上述贮备液，最终浓度为 0.6-2.4 U/mL。请注意，不建议浓度高于 2.4 U/mL。为获得最佳结果，使用 0.22μm 过滤器膜。
储存条件（工作溶液）：-15°C 至-25°C
复溶贮备液在 2 ~ 8°C 下可稳定保存 2 周。储存长达 2 个月时，贮备液应等分冷冻。避免反复冻融。
用 PBS 稀释的工作液在 2～8°C 可稳定保存 3 天。
储存及稳定性
其他说明
法律信息
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(F)
does not flash
闪点(C)
does not flash
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