Merck
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10108057001

Roche

胃蛋白酶

lyophilized (salt-free), ~2500 units/mg protein (At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.)

Synonym(s):
胃蛋白酶
Enzyme Commission number:

生物来源

pig gastric mucosa

质量水平

100

形式

lyophilized (salt-free)

specific activity

~2500 units/mg protein (At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.)

包装

pkg of 1 g

manufacturer/tradename

Roche

最佳pH

1.8-2.2

运输

wet ice

储存温度

2-8°C

一般描述

胃蛋白酶是一种来自猪胃粘膜的天冬氨酸内蛋白酶。它主要用于对酸性介质中的蛋白质和多肽进行非特异性水解,还能促进天然免疫球蛋白的限制性酶解,产生生物活性片段。

特异性

天冬氨酸内肽酶具有相对广泛的特异性。优先裂解:P1和P1′ 位置的疏水性残基,其中芳香族优先。

应用

胃蛋白酶已用于消化大鼠肝脏组织。[1]

制备说明

工作浓度:0.05至0.5 mg/ml
储存条件(工作溶液):在中性pH和无菌条件下,胃蛋白酶溶液可以在2至8℃下稳定储存至少1周。冷冻的酶溶液分装品可能更稳定。

溶剂为10mM HCl(5-10mg/ml)的储备溶液也可以在2至8℃下稳定储存至少1周。避光、密闭保存。

在40℃下短暂(2分钟)孵育对冻干胃蛋白酶或胃蛋白酶溶液的活性的影响可忽略不计。

其他说明

仅用于生命科学研究。不可用于诊断。

象形图

Exclamation markHealth hazard

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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