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一般說明
链霉亲和素与碱性磷酸酶 (AP) 共价偶联,用于制备链霉亲和素-AP 偶联物。
碱性磷酸酶
碱性磷酸酶常被用作酶免疫测定的标记物。AP 将敏感底物 X-磷酸盐/NBT 转化为稳定的水溶性产物。
碱性磷酸酶
碱性磷酸酶常被用作酶免疫测定的标记物。AP 将敏感底物 X-磷酸盐/NBT 转化为稳定的水溶性产物。
應用
链霉亲和素-AP 已用于检测生物素化化合物,用于:
- ELISA
- 免疫组织化学
- 蛋白印迹
外觀
溶液,在 50 mM 三乙醇胺缓冲液、3 MNaCl、1 mM MgCl 2 、0.1 mM ZnCl 2 、10 mg/mL 牛血清白蛋白 (pH 7.6) 中稳定 。
準備報告
工作溶液: 50-1,000 mU/ml(即贮备液可稀释)。将缀合物储备溶液在 100 mM Tris-HCl、150 mM NaCl (pH 7.5) 中稀释。
工作浓度: 缀合物的工作浓度取决于应用和底物。标示浓度应作为指导。
ELISA:50-200 mU/ml(1:5000-1:20,000 稀释)
免疫组织化学/细胞化学:250-1,000 mU/ml(1:1000-1:4000 稀释)
免疫印迹:200-500 mU/ml(1:2000-1:5000 稀释)
稳定性: 浓缩储备液在 2 ~ 8°C 下可稳定储存至标签上打印的有效期为止。 切勿冻结!
工作浓度: 缀合物的工作浓度取决于应用和底物。标示浓度应作为指导。
ELISA:50-200 mU/ml(1:5000-1:20,000 稀释)
免疫组织化学/细胞化学:250-1,000 mU/ml(1:1000-1:4000 稀释)
免疫印迹:200-500 mU/ml(1:2000-1:5000 稀释)
稳定性: 浓缩储备液在 2 ~ 8°C 下可稳定储存至标签上打印的有效期为止。 切勿冻结!
其他說明
仅用于生命科学研究。不可用于诊断。
相關產品
訊號詞
Warning
危險聲明
危險分類
Skin Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
No data available
閃點(°C)
No data available
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