Merck

11089161001

Roche

链霉抗生物素蛋白-AP缀合物

solution, pkg of 1 mL (1,000U)

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别名:
链霉亲和素

形式

solution

质量水平

包装

pkg of 1 mL (1,000U)

manufacturer/tradename

Roche

储存温度

2-8°C

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此商品
S3887S289011093266910
storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

packaging

pkg of 1 mL (1,000U)

packaging

-

packaging

-

packaging

pkg of 200 μL (150U)

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Roche

一般描述

链霉亲和素与碱性磷酸酶 (AP) 共价偶联,用于制备链霉亲和素-AP 偶联物。

碱性磷酸酶
碱性磷酸酶常被用作酶免疫测定的标记物。AP 将敏感底物 X-磷酸盐/NBT 转化为稳定的水溶性产物。

应用

链霉亲和素-AP 已用于检测生物素化化合物,用于:
  • ELISA
  • 免疫组织化学
  • 蛋白印迹

外形

溶液,在 50 mM 三乙醇胺缓冲液、3 MNaCl、1 mM MgCl 2 、0.1 mM ZnCl 2 、10 mg/mL 牛血清白蛋白 (pH 7.6) 中稳定

制备说明

工作溶液: 50-1,000 mU/ml(即贮备液可稀释)。将缀合物储备溶液在 100 mM Tris-HCl、150 mM NaCl (pH 7.5) 中稀释。

工作浓度: 缀合物的工作浓度取决于应用和底物。标示浓度应作为指导。

ELISA:50-200 mU/ml(1:5000-1:20,000 稀释)
免疫组织化学/细胞化学:250-1,000 mU/ml(1:1000-1:4000 稀释)
免疫印迹:200-500 mU/ml(1:2000-1:5000 稀释)

稳定性: 浓缩储备液在 2 ~ 8°C 下可稳定储存至标签上打印的有效期为止。 切勿冻结!

其他说明

仅用于生命科学研究。不可用于诊断。

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警示用语:

Warning

危险声明

危险分类

Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

No data available

闪点(°C)

No data available


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