Merck
All Photos(1)

11284932001

Roche

DNase I

from bovine pancreas

Synonym(s):
Dnase
Enzyme Commission number:

生物来源

bovine pancreas

质量水平

形式

lyophilized

包装

pkg of 100 mg

manufacturer/tradename

Roche

technique(s)

cell culture | mammalian: suitable

储存温度

2-8°C

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This Item
AMPD11010415900104536282001
DNase I from bovine pancreas

Roche

11284932001

DNase I

DNase I Amplification Grade

Sigma-Aldrich

AMPD1

脱氧核糖核酸酶I

DNase I grade II, from bovine pancreas

Roche

10104159001

DNase I

DNase I recombinant grade I, from bovine pancreas, expressed in Pichia pastoris

Roche

04536282001

重组DNase I

biological source

bovine pancreas

biological source

-

biological source

bovine pancreas

biological source

-

form

lyophilized

form

liquid

form

lyophilized

form

lyophilized

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

Roche

manufacturer/tradename

Roche

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

DNA extraction: suitable

technique(s)

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

一般描述

DNase I是一种可降解DNA的双链特异性内切核酸酶。牛胰腺脱氧核糖核酸酶I(DNase I)是DNA小沟相互作用的核酸酶,特异性相对较低。该蛋白质由两个中心β折叠构成,而每个折叠由六个β-链所组成。该结构被大量的环以及α-螺旋区域包围。该酶与核酸外切酶III具有结构相似性。DNase I是研究得最透彻的哺乳动物来源的内切酶之一。

生化/生理作用

在组织解离过程中,部分细胞被裂解并导致DNA的释放。单分子DNA可能会导致细胞结团。向解离缓冲液中加入DNase I会导致该细胞外DNA的降解,因而避免了因意外结团造成的细胞损失。

单位定义

一个单位表示在测定条件下导致吸光度以0.001每分钟的速率而增加的酶活力。

制备说明

激活剂:二价金属离子 (Ca2+, Mg2+)
工作浓度:0.01 至 1 mg/ml
注意:必须针对每种细胞类型单独确定工作浓度。为实现最佳活性,酶须添加 5 mM Mg2+

重悬

用无菌的双蒸水对冻干酶进行重悬(10 mg/ml);用PBS(磷酸盐缓冲液)、HBSS(Hank′s 平衡盐溶液)或培养基进行进一步的稀释。

其他说明

仅用于生命科学研究。不用于诊断操作。

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(F)

does not flash

闪点(C)

does not flash

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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Product Information Sheet

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