11814524001

Roche

rGFP

Green Fluorescent Protein from jellyfish Aequorea victoria, recombinant expressed in E. coli

别名:
绿色荧光蛋白, gfp,r-

质量水平

100

形式

solution

包装

pkg of 50 μL (1 mg/ml)

manufacturer/tradename

Roche

运输

wet ice

储存温度

2-8°C

一般描述

从水母维多利亚多管发光水母分离的 GFP 表现出自发荧光特性。它是各种异源系统中基因表达的新型重要标记。它稳定存在于植物细胞中,并具有光漂白。GFP 的分子克隆产生具有荧光激发和发射光谱的融合蛋白。

特异性

热灭活:荧光将通过还原剂(例如 DTT 或二硫代硫酸钠)淬灭,并被过氧化物完全抑制。
极端 pH 条件(pH< 4 and pH > 11)以及乙醇含量高于 60% 抑制荧光。

应用

重组绿色荧光蛋白(rGFP)也已用于间接酶联免疫吸附测定。
重组绿色荧光蛋白(rGFP)适合作为:
  • 通过 SDS-PAGE 检测 GFP 融合蛋白时的阳性对照。
  • 使用抗 GFP 单克隆抗体通过蛋白质印迹检测 GFP 融合蛋白时的阳性对照。
  • 用于涉及荧光显微镜的实验的标准。

外形

溶液,1mg/ml rGFP 的 5 mM Tris-HCl,5 mM EDTA,pH 8.0

制备说明

工作浓度:推荐用于 rGFP 的量:
  • SDS-PAGE(考马斯染色):1 μg
  • 蛋白质印迹:5 ng

其他说明

仅用于生命科学研究。不可用于诊断。
发射最大值: 509nm
最大激发:395nm

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 1

闪点(F)

No data available

闪点(C)

No data available

分析证书

原产地证书 (CofO)

Efficient central nervous system AAVrh10-mediated intrathecal gene transfer in adult and neonate rats.
Hordeaux J, et al.
Gene Therapy, 22(4), 316-316 (2015)
S Inouye et al.
FEBS letters, 341(2-3), 277-280 (1994-03-21)
Expression of the cDNA for Aequorea green fluorescent protein in E. coli yielded a fused protein with fluorescence excitation and emission spectra virtually identical to those of the native green fluorescent protein. Further, a solution of the protein, when mixed...
Methods for addressing the protein-protein interaction between histone deacetylase 6 and ubiquitin
dos Santos Passos C, et al.
bioRxiv, 203372-203372 (2017)
J Sheen et al.
The Plant journal : for cell and molecular biology, 8(5), 777-784 (1995-11-01)
The green-fluorescent protein (GFP) from jellyfish Aequorea victoria has been used as a convenient new vital marker in various heterologous systems. However, it has been problematic to express GFP in higher eukaryotes, especially in higher plants. This paper reports that...
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