Merck

CK-RO

Roche

肌酸激酶(CK)

from rabbit muscle

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别名:
肌酸磷酸激酶

生物来源

rabbit muscle

质量水平

形式

lyophilized

specific activity

~350 units/mg protein (at 25 °C (800 U/mg lyophilizate at 37 °C) with creatine phosphate and ADP as the substrates, and N-acetyl-L-cysteine as the activator.)

包装

pkg of 100 mg (10127566001)
pkg of 500 mg (10736988001)

manufacturer/tradename

Roche

颜色

white to light yellow

最佳pH

6.0-7.0(reverse reaction)
9.0(forward reaction)

溶解性

water: soluble

适用性

suitable for immunoprecipitation

NCBI登记号

UniProt登记号

application(s)

life science and biopharma
sample preparation

异质活性

ATPase <0.001%
HK <0.001%
myokinase <0.001%

储存温度

2-8°C

Gene Information

rabbit ... CKM(100009056)

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MAK116C0984SAB1410202
Creatine Kinase (CK) from rabbit muscle

Roche

CK-RO

肌酸激酶(CK)

form

lyophilized

form

-

form

liquid

form

buffered aqueous solution

specific activity

~350 units/mg protein (at 25 °C (800 U/mg lyophilizate at 37 °C) with creatine phosphate and ADP as the substrates, and N-acetyl-L-cysteine as the activator.)

specific activity

-

specific activity

-

specific activity

-

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

color

white to light yellow

color

-

color

-

color

-

optimum pH

6.0-7.0(reverse reaction), 9.0(forward reaction)

optimum pH

-

optimum pH

-

optimum pH

-

一般描述

ATP:肌 N-磷酸转移酶
肌酸激酶(CK)的摩尔质量为85kDa。它在细胞溶质形式以二聚体存在,并且在线粒体形式以二聚体和八聚体存在。细胞溶质形式命名为MM,BB和MB,其中M和B分别对应肌肉和脑。来自兔肌肉的CK是含有两个380个氨基酸的相同多肽链的二聚体。每个亚基具有四个半胱氨酸,Cys73、Cys145、Cys253和Cys282,但缺少链间和链内二硫键。

应用

肌酸激酶已被用作体外翻译测定中使用的翻译缓冲液的组分,并且作为能量再生混合物的组分。

生化/生理作用

肌酸激酶(CK)是一种胍基激酶,消耗ATP催化肌酸可逆磷酸化为磷酸肌酸。它在具有高能量需求的细胞中快速再生ATP中起着至关重要的作用。人血液中CK水平的增加表明神经系统和心肌疾病、恶性低温和某些肿瘤。金属(例如Mg、Mn和Ca)充当CK的辅助因子。它还形成蛋白激酶C的底物,蛋白激酶C是参与细胞生长的重要信号分子。人们发现 CK的酶促反应产物,磷酸肌酸,可以阻止 HIV(人类免疫缺陷病毒)在巨噬细胞中复制。

质量

污染物:&lt;0.001% ATP酶,HK和肌激酶,每种

产品规格

具体活动:在+ 25°C下大约 350U/mg冻干物(+ 37°C下约800U/mg 冻干物),其中磷酸肌酸和ADP作为底物,并且N-乙酰基-L-半胱氨酸作为活化剂。

单位定义

一个单位CK活性(+ 25°C)= 2.3 U(+ 37°C),肌酸蛋白-P和ADP 作为底物,NAcCys作为重活化剂。

外形

冻干粉,稳定

制备说明

储存条件(工作液):常规应用中的工作溶液和储存条件:
溶于0.1M 咪唑缓冲液中;等分并储存在- 15至-25°C。
在-15至-25°C稳定约四周。
避免反复冻融。

溶于0.5% NaHCO3溶液中。
不要冷冻。避光储存。
在2至8°C条件下储存时,稳定2-3天。

用含有0.2mM DTT的30mM甘氨酸缓冲液(pH9.0)溶解。
在2至8°C条件下储存时,稳定2-3天。

其他说明

仅用于生命科学研究。不可用于诊断。

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

does not flash

闪点(°C)

does not flash


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其他客户在看

Irina M Kuznetsova et al.
Biochimica et biophysica acta, 1596(1), 138-155 (2002-05-02)
GdmCl-induced unfolding of rabbit muscle creatine kinase, CK, has been studied by a variety of physico-chemical methods including near and far UV CD, SEC, intrinsic fluorescence (intensity, anisotropy and lifetime) as well as intensity and lifetime of bound ANS fluorescence.
Aurélie M Rakotondrafara et al.
Nature protocols, 6(5), 563-571 (2011-04-30)
Much of the regulation of gene expression occurs at the level of protein synthesis. In addition to the canonical translation factors, a multitude of proteins and microRNAs (miRNAs) act as regulatory trans-acting factors. Mechanistic analysis of translational control benefits from
Marta Teperek et al.
International journal of molecular sciences, 15(9), 16719-16740 (2014-09-23)
Spermatozoa are more efficient at supporting normal embryonic development than spermatids, their immature, immediate precursors. This suggests that the sperm acquires the ability to support embryonic development during spermiogenesis (spermatid to sperm maturation). Here, using Xenopus laevis as a model
J K Rao et al.
FEBS letters, 439(1-2), 133-137 (1998-12-16)
The crystal structure of rabbit muscle creatine kinase, solved at 2.35 A resolution by X-ray diffraction methods, clearly identified the active site with bound sulfates surrounded by a constellation of arginine residues. The putative binding site of creatine, which is
Marta Teperek et al.
Genome research, 26(8), 1034-1046 (2016-04-02)
For a long time, it has been assumed that the only role of sperm at fertilization is to introduce the male genome into the egg. Recently, ideas have emerged that the epigenetic state of the sperm nucleus could influence transcription

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