所有图片(1)

COLLP-RO

Roche

胶原酶P

from Clostridium histolyticum

别名:
collagenase

质量水平

100

无菌性

non-sterile

形式

lyophilized

包装

pkg of 1 g (11249002001)
pkg of 100 mg (11213857001)
pkg of 2.5 g (11213873001)
pkg of 500 mg (11213865001)

运输

wet ice

储存温度

2-8°C

一般描述

从一种特殊的Clostridium histolyticum菌株细胞外培养滤液中制备酶混合物,胶原酶P。胶原酶P是一种研究用生物化学物,生产不遵循US良好生产规范(GMP)规范。
注意:胶原酶是一种 Clostridium histolyticum培养悬浮的非灭菌制备。它不可用于分离胰岛,移植进人体。

特异性

酶活性:
胶原酶活性:>1.5U/mg (根据Wünsch) (+25℃, 4-苯基-叠氮苯甲基-氧羟基-Pro-Leu-Gly-Pro-D-Arg作为底物)
污染酶活性:胰蛋白酶,梭菌蛋白酶和总蛋白水解酶活性
胶原酶P有很高胶原酶活性(>1.5U/mg),可用于小鼠胰岛分离。
特异性:胶原酶可降解天然胶原蛋白。梭菌蛋白酶、胰蛋白酶样酶和中性蛋白酶也会降解其他蛋白质。

应用

来自C. histolyticum胶原酶被用来分解组织,建立原代细胞培养。因为胶原酶P有高胶原活性,特别适合从小鼠和大鼠(功能测试)分离胰岛。制备产物适合从大鼠附睾脂肪垫分离脂肪细胞。

特点和优势

产品:
冻干粉,非灭菌

制备说明

激活剂:Ca2+
抑制剂:
胶原酶抑制剂:EDTA, EGTA, Cys, His, DTT, 2-巯基乙醇
胶原酶不会被血清所抑制。
梭菌蛋白酶抑制剂:TLCK
胰蛋白酶抑制剂:抑肽酶、胰蛋白酶抑制剂(蛋清、大豆)
工作浓度:0.5到2.5mg/ml
从小鼠和大鼠胰腺中分离0.5到1.5mg/ml胰岛
脂肪细胞中分离大约2mg/ml
储存条件(工作溶液):-15到-25℃
重组溶液在-15到-25℃稳定。准备合适的组分,避免反复冻融。

重悬

可重悬于任意平衡盐溶液(如,HBSS)

其他说明

仅用于生命科学研究。不可用于诊断。

象形图

Exclamation markHealth hazard

警示用语:

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

闪点(F)

does not flash

闪点(C)

does not flash

分析证书

原产地证书 (CofO)

T-cadherin (Cdh13) in association with pancreatic ?-cell granules contributes to second phase insulin secretion.
Tyrberg B
Islets, 3(6), 327-337 (2011)
Luigi Racioppi et al.
The Journal of biological chemistry, 287(14), 11579-11591 (2012-02-16)
Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) plays a key role in regulating food intake and energy expenditure at least in part by its actions in hypothalamic neurons. Previously, we showed that loss of CaMKK2 protected mice from high-fat diet (HFD)-induced obesity...
Fumin Lin et al.
Endocrinology, 152(10), 3668-3679 (2011-08-25)
When fed a standard chow diet, CaMKK2 null mice have increased adiposity and larger adipocytes than do wild-type mice, whereas energy balance is unchanged. Here, we show that Ca(2+)/calmodulin-dependent protein kinase kinase 2 (CaMKK2) is expressed in preadipocytes, where it...

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