ENDOLYSS-RO

Roche

蛋白内切酶Lys-C 测序级

from Lysobacter enzymogenes

形式

lyophilized

specific activity

≥200 units/mg protein (at 25 °C, with Chromozym PL)

分子量

33 kDa by SDS-PAGE (reducing)

包装

pkg of 3 × 5 μg (11047825001)
pkg of 5 μg (11420429001)

manufacturer/tradename

Roche

最佳pH

8.5-8.8

pH范围

5 - 12

运输

wet ice

储存温度

2-8°C

一般描述

内蛋白酶赖氨酸-碳测序等级是从产酶溶血杆菌中分离出来的,是一种高度纯化和特异的蛋白酶。

特异性

这种酶是一种丝氨酸蛋白酶,能特异性水解赖氨酸羧基侧的酰胺、酯和肽键(在三-盐酸缓冲液中,pH 7.0至9.0)。
热失活:酶可以通过加热(100℃下5分钟)或TCA变性。

应用

使用内蛋白酶赖氨酸-碳测序等级进行蛋白质结构分析和序列分析。它适用于消化溶液、聚丙烯酰胺凝胶或吸水膜中的蛋白质。

制备说明

储存条件(工作溶液):+2至+8℃

重悬

冻干内蛋白酶赖氨酸-碳测序等级在50 & # 956;L双蒸水中重构。生成缓冲液浓度为50 mM Hepes、pH 8.0、10 mM乙二胺四乙酸和5 mg/ml棉子糖。为避免自溶,培养温度不应超过37℃。
在4 M尿素中稳定。

其他说明

仅用于生命科学研究。不可用于诊断。

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 2

闪点(F)

does not flash

闪点(C)

does not flash

分析证书

原产地证书 (CofO)

Tao Xu et al.
Nature protocols, 4(3), 325-332 (2009-02-21)
This protocol outlines the essential steps of mass spectrometry-based analysis of protein samples that can be used to identify post-translational arginylation. We describe special considerations for sample preparation and digestion, mass spectrometry analysis using high-precision instruments, database searching for the...
H Alexander Ebhardt et al.
Rapid communications in mass spectrometry : RCM, 28(24), 2735-2743 (2014-11-08)
Tandem mass (MS/MS) spectra generated by collision-induced dissociation (CID) typically lack redundant peptide sequence information in the form of e.g. b- and y-ion series due to frequent use of sequence-specific endopeptidases cleaving C- or N-terminal to Arg or Lys residues....
Jung Hun Oh et al.
Journal of proteome research, 10(3), 1406-1415 (2011-01-14)
Many efforts have been made to discover novel bio-markers for early disease detection in oncology. However, the lack of efficient computational strategies impedes the discovery of disease-specific biomarkers for better understanding and management of treatment outcomes. In this study, we...
Jason A Wall et al.
American journal of physiology. Heart and circulatory physiology, 291(5), H2462-H2472 (2006-06-13)
Ischemia-reperfusion (I/R) has critical consequences in the heart. Recent studies on the functions of I/R-activated kinases, such as p38 mitogen-activated protein kinase (MAPK), showed that I/R injury is reduced in the hearts of transgenic mice that overexpress the p38 MAPK...

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