Merck

PMSF-RO

Roche

PMSF

Phenylmethylsulfonyl fluoride

登录to View Organizational & Contract Pricing

Select a Size

别名:
苯甲磺酰氟, 苯甲基磺酰氟, α-甲苯磺酰氟, PMSF, 苄磺酰氟
Empirical Formula (Hill Notation):
C7H7FO2S
CAS号:
分子量:
174.19
Beilstein:
2088311
MDL编号:
PubChem化学物质编号:

质量水平

检测方案

98% (TLC)

形式

solid

分子量

174.2

包装

pkg of 10 g (10837091001)
pkg of 25 g (11359061001)

manufacturer/tradename

Roche

浓度

17-170 μg/mL

technique(s)

western blot: suitable

pKa 

7.0

mp

92-94 °C

溶解性

>10 mg/mL (Soluble to >10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at +25°C.)

运输

ambient

储存温度

20-25°C

SMILES string

FS(=O)(=O)Cc1ccccc1

InChI

1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2

InChI key

YBYRMVIVWMBXKQ-UHFFFAOYSA-N

正在寻找类似产品? Visit 产品对比指南

比较类似商品

查看完整比较结果

显示差异

1 of 4

此商品
P76265233278830
PMSF Phenylmethylsulfonyl fluoride

Roche

PMSF-RO

PMSF

Phenylmethanesulfonyl fluoride ≥98.5% (GC)

Sigma-Aldrich

P7626

苯甲磺酰氟

Phenylmethylsulfonyl Fluoride Phenylmethylsulfonyl Fluoride, CAS 329-98-6, is an irreversible inhibitor of serine proteases. It causes sulfonylation of the active-site serine residues.

Sigma-Aldrich

52332

苯甲基磺酰氟

Phenylmethanesulfonyl fluoride ≥99.0% (T)

Sigma-Aldrich

78830

苯甲磺酰氟

form

solid

form

powder

form

crystalline solid

form

powder or crystals

mol wt

174.2

mol wt

-

mol wt

-

mol wt

-

mp

92-94 °C

mp

91-94 °C

mp

-

mp

92-95 °C

solubility

>10 mg/mL (Soluble to >10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at +25°C.)

solubility

dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.), H2O: unstable

solubility

ethanol: soluble, isopropanol: soluble, methanol: soluble

solubility

anhydrous isopropanol: soluble 35 mg/mL, clear to faintly hazy, colorless to faintly yellow (Heating required)

shipped in

ambient

shipped in

-

shipped in

ambient

shipped in

-

特异性

可抑制丝氨酸蛋白酶,如胰凝乳蛋白酶、胰蛋白酶、凝血酶和半胱氨酸蛋白酶木瓜蛋白酶(通过DTT处理可逆)。不抑制金属蛋白酶、大多数的半胱氨酸蛋白酶或天冬氨酸蛋白酶。

应用

PMSF(C7H7O2SF)可抑制丝氨酸蛋白酶,如胰凝乳蛋白酶、胰蛋白酶、凝血酶和半胱氨酸蛋白酶木瓜蛋白酶(通过DTT处理可逆)。它不抑制金属蛋白酶、大多数的半胱氨酸蛋白酶或天冬氨酸蛋白酶。
PMSF(苯甲基磺酰氟)已用于在进行Western blotting之前从组织和细胞中制备蛋白质提取物。

生化/生理作用

PMSF(苯甲基磺酰氟)可作为多种蛋白酶和其他酶的非特异性抑制剂,如棕榈酰辅酶A脱酰酶、芳基硫酸酯酶A、乙酰胆碱酯酶、胰蛋白酶和胰凝乳蛋白酶。它是一种不可逆的抑制剂,且丝氨酸残基的磺酰化羟基存在于酶的活性位点中。

质量

纯度:色谱均相的

制备说明

激活剂:PK需要Mg2+ (o或Mn2+, CO2+)以及K+ (或NH4+, Rb+)以实现完全的活性。
工作浓度:17至170 μg/ml (0.1至1 mM)
工作溶液:由于PMSF的短半衰期,必须在每一步的纯化步骤中加入新鲜的溶液。在某些特定条件如碱性pH值下,PMSF的半衰期可能只有35分钟。常用于沉淀蛋白的高盐条件(3M)可能也会逆转PMSF的抑制。Pefabloc SC被推荐作为一种无毒、水溶性且水稳定的替代品。
储存条件(工作溶液):15至25 °C
在15至25 °C的100%异丙醇中的PMSF储备溶液可稳定至少9个月。

重悬

可在异丙醇、乙醇、甲醇或1,2-丙二醇中达到> 10 mg/ml的溶解度。在水相溶液中不稳定。在15至25 °C的100%异丙醇中可稳定至少9个月。

其他说明

仅用于生命科学研究。不可用于诊断。
由于短的半衰期,必须在每一步的纯化步骤中加入新鲜的溶液。在某些特定条件如碱性pH值下,PMSF的半衰期可能只有35分钟。常用于沉淀蛋白的高盐条件(3M)可能也会逆转PMSF的抑制。Pefabloc SC被推荐作为一种无毒、水溶性且水稳定的替代品。

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题?欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ (Lot或Batch)字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

  • 如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。

  • 如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。

未找到您寻找的产品?

部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。

索取COA

其他客户在看

Slide 1 of 9

1 of 9

Sodium orthovanadate ≥90% (titration)

Sigma-Aldrich

S6508

正钒酸钠

Pefabloc® SC analytical standard

Supelco

76307

Pefabloc® SC

Pepstatin A microbial, ≥90% (HPLC)

Sigma-Aldrich

P5318

胃酶抑素 A

Pepstatin from Streptomyces species

Roche

PEPS-RO

胃蛋白酶抑制剂

Benzamidine ≥95.0%

Sigma-Aldrich

12072

苯甲脒

Sodium orthovanadate 99.98% trace metals basis

Sigma-Aldrich

450243

正钒酸钠

D R Compton et al.
The Journal of pharmacology and experimental therapeutics, 283(3), 1138-1143 (1998-02-12)
Anandamide is an putative endogenous cannabinoid ligand that produces pharmacological effects similar to those of Delta9-tetrahydrocannabinol, the principle psychoactive constituent in marijuana. There is considerable evidence that the enzyme inhibitor phenylmethylsulfonyl fluoride (PMSF) is capable of altering the actions of
Hongjuan Yu et al.
PloS one, 8(11), e80815-e80815 (2013-11-16)
A kinase interacting protein 1 (AKIP1) is a molecular regulator of protein kinase A and nuclear factor kappa B signalling. Recent evidence suggests AKIP1 is increased in response to cardiac stress, modulates acute ischemic stress response, and is localized to
Guilian Tian et al.
The Journal of biological chemistry, 284(28), 18980-18993 (2009-05-09)
Clarin-1 is the protein product encoded by the gene mutated in Usher syndrome III. Although the molecular function of clarin-1 is unknown, its primary structure predicts four transmembrane domains similar to a large family of membrane proteins that include tetraspanins.
Ahmed M Kotb et al.
Breast cancer research : BCR, 13(5), R104-R104 (2011-10-28)
E-cadherin (E-cad; cadherin 1) and N-cadherin (N-cad; cadherin 2) are the most prominent members of the cadherin family of cell adhesion molecules. Although they share many structural and functional features, they are expressed in an almost mutually exclusive manner in

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门