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PROTAA-RO

Roche

蛋白A琼脂糖

>98% (HPLC and SDS-PAGE), suspension

别名:
琼脂糖, 蛋白A

测定

>98% (HPLC and SDS-PAGE)

形式

suspension

包装

pkg of 15 mL (05015979001 [settled resin volume])
pkg of 2 mL (11719408001 [settled resin volume])
pkg of 5 mL (11134515001 [settled resin volume])

容量

>18 mg/mL binding capacity (human IgG (polyclonal))(the IgG is loaded at pH 7.4 and eluted with 200 mM glycine, pH 2.0)

运输

wet ice

储存温度

2-8°C

一般描述

重组蛋白A在蛋白A琼脂糖生产中的应用具有以下优点:它完全不含已知具有促有丝分裂作用的葡萄球菌肠毒素(分离自金黄色葡萄球菌的蛋白A中的污染物) 。固定化学可在广泛的pH和缓冲条件下提供高IgG的结合能力以及防漏稳定性。根据洗脱条件,凝胶可以使用30次以上。

蛋白A泄漏:<18 ng Protein A/ml (ELISA)
Regeneration: the gel can be used approximately 30 times
Structure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98%纯度,HPLC,SDS-Page/1ml凝胶
蛋白A,固定化。

特异性

蛋白A是从特定细菌菌株中分离的细胞壁蛋白,其具有来自不同物种的某些类免疫球蛋白的特异性结合位点。蛋白A可在不同程度结合IgM、IgA、IgD和大多数IgG亚类。

应用

从腹水或细胞培养上清液中纯化小鼠单克隆抗体。蛋白A或蛋白G琼脂糖亲和基质可用于从粗细胞提取物中纯化抗体 以及蛋白质的免疫沉淀或共免疫沉淀。它还被用于光活化-核糖核苷-增强的交联免疫沉淀(PAR-CLIP)分析。

组分

Preswollen凝胶,即用型,2ml或5ml(沉淀凝胶体积)悬浮于缓冲液中的凝胶,该缓冲液中含有20%乙醇作为保藏剂,非无菌。
蛋白A含量:3mg/ml预溶胀凝胶

质量

纯度:> 98%(HPLC,SDS-PAGE);完全不含葡萄球菌肠毒素。

其他说明

仅用于生命科学研究。不可用于诊断。

象形图

FlameExclamation mark

警示用语:

Warning

危险声明

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

闪点(F)

102.2 °F

闪点(C)

39 °C

分析证书

原产地证书 (CofO)

David Llobet-Navas et al.
Molecular and cellular biology, 34(23), 4216-4231 (2014-10-01)
Recently, we demonstrated that the microRNA 424(322)/503 [miR-424(322)/503] cluster is transcriptionally controlled by transforming growth factor β (TGF-β) in the mammary epithelium. Induction of this microRNA cluster impacts mammary epithelium fate by regulating apoptosis and insulin-like growth factor 1 (IGF1)...
R Travis Taylor et al.
Methods (San Diego, Calif.), 55(2), 166-171 (2011-08-23)
Ubiquitin (Ub) conjugation to a substrate protein is a widely used cellular mechanism for control of protein stability and function, modulation of signal transduction pathways and antiviral responses. Identification and characterization of ubiquitinated viral proteins is an important step in...
Hongbo Yang et al.
Developmental biology, 404(2), 49-60 (2015-05-26)
Angiogenesis relies on specialized endothelial tip cells to extend toward guidance cues in order to direct growing blood vessels. Although many of the signaling pathways that control this directional endothelial sprouting are well known, the specific cellular mechanisms that mediate...
Ji-An Pan et al.
Molecular and cellular biology, 31(15), 3158-3170 (2011-06-02)
The accumulation of damaged or misfolded proteins, if unresolved, can lead to a detrimental consequence within cells termed proteotoxicity. Since cancerous cells often display elevated protein synthesis and by-product disposal, inhibition of the protein degradation pathways is an emerging approach...
Daniel J Ryder et al.
PloS one, 10(8), e0136247-e0136247 (2015-08-25)
Skeletal muscle atrophy is a consequence of several physiological and pathophysiological conditions including muscle disuse, aging and diseases such as cancer and heart failure. In each of these conditions, the predominant mechanism contributing to the loss of skeletal muscle mass...

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