Merck
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RSTREP-RO

Roche

链霉亲和素，重组

lyophilized, ≥17 units/mg protein, pH range 5.0-9.0

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别名:
链亲和素

形式

lyophilized

质量水平

100

specific activity

≥17 units/mg protein

分子量

~52 kDa

包装

pkg of 1 mg (11721666001)
pkg of 5 mg (11721674001)

manufacturer/tradename

Roche

pH range

5.0-9.0

pI 

7.4-7.7 (two main bands)

溶解性

water: 10 mg/L (mA405=100)

储存温度

2-8°C

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此商品
027440926011093266910
Streptavidin, recombinant lyophilized, ≥17 units/mg protein, pH range 5.0-9.0

Roche

RSTREP-RO

链霉亲和素，重组

Sigma-Aldrich

Sigma-Aldrich

02744

Atto 655-Streptavidin

Sigma-Aldrich

Sigma-Aldrich

09260

Atto 425-Streptavidin

Streptavidin-AP-conjugate for nucleic acid detection

Roche

11093266910

Streptavidin-AP-conjugate for nucleic acid detection

specific activity

≥17 units/mg protein

specific activity

-

specific activity

-

specific activity

-

mol wt

~52 kDa

mol wt

-

mol wt

-

mol wt

-

packaging

pkg of 1 mg (11721666001), pkg of 5 mg (11721674001)

packaging

-

packaging

-

packaging

pkg of 200 μL (150U)

solubility

water: 10 mg/L (mA405=100)

solubility

DMF: soluble, DMSO: soluble, acetonitrile: soluble

solubility

H2O: soluble

solubility

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

一般描述

Streptavidin, a highly versatile protein, is naturally produced by Streptomyces avidinii. It exhibits a strong affinity for biotin, forming a non-covalent yet robust binding interaction. This unique property makes streptavidin an ideal choice for various biological detection systems, including western blotting, enzyme-linked immunosorbent assays (ELISA), and immunohistochemistry.

应用

使用重组链霉亲和素包被固相（，例如 、微孔板、微球 等 ）以及通用免疫学检测系统（链霉亲和素/生物素系统）中的生物素标记半抗原和抗体。

序列

链霉亲和素是一种分子量约为 53kd 的四聚体，由约 13kd 的四个亚基组成，每个亚基含有单个生物素结合位点.
每个亚基有 6 个酪氨酸残基。这种蛋白质不含碳水化合物。

单位定义

一个单元结合 1μg pH 8.9 的 D-生物素。

外形

白色冻干粉；在 20 mM 磷酸钾 pH 6.5 溶液中冻干
蛋白： 0.7±0.1 mg 蛋白冻干品 (A 282 = 3.1)
KD: 10-15M

重悬

加入 1 mL 双蒸水，使最终浓度分别为 1 mg/mL (11 721 666 001) 或 5 mg/mL (11 721 674 001)。

其他说明

仅用于生命科学研究。不可用于诊断。

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(F)

does not flash

闪点(C)

does not flash

Certificates of Analysis (COA)

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索取COA

Automated combinatorial process for nanofabrication of structures using bioderivatized nanoparticles.
Dehlinger D, et al.
Journal of the Association for Laboratory Automation, 5, 267-276 (2007)
John E G McCarthy et al.
Methods in enzymology, 430, 247-264 (2007-10-05)
A growing number of biophysical techniques use immobilized reactants for the quantitative study of macromolecular reactions. Examples of such approaches include surface plasmon resonance, atomic force microscopy, total reflection fluorescence microscopy, and others. Some of these methods have already been
THE PROPERTIES OF STREPTAVIDIN, A BIOTIN-BINDING PROTEIN PRODUCED BY STREPTOMYCETES.
L CHAIET et al.
Archives of biochemistry and biophysics, 106, 1-5 (1964-07-20)

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