Merck

63069

Sigma-Aldrich

氯化镁 溶液

BioUltra, for molecular biology, ~1 M in H2O

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别名:
Magnesium Chloride hydrate, Magnesium chloride (MgCl2)hexahydrate, MgCl2
线性分子式:
MgCl2
CAS号:
分子量:
95.21
MDL编号:
PubChem化学物质编号:
NACRES:
NA.26

等级

for molecular biology

质量水平

产品线

BioUltra

形式

liquid

浓度

~1 M in H2O

杂质

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.0002% total Nitrogen (N)

pH值(酸碱度)

5.0-7.5 (25 °C, 1 M in H2O)

溶解性

water: soluble

密度

1.08 g/mL at 20 °C

痕量阴离子

phosphate (PO43-): ≤10 mg/kg
sulfate (SO42-): ≤50 mg/kg

痕量阳离子

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤10 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤50 mg/kg
Li: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤500 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

1 M in H2O

紫外吸收

λ: 260 nm Amax: 0.05
λ: 280 nm Amax: 0.03

SMILES string

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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Magnesium chloride solution BioUltra, for molecular biology, ~1 M in H2O

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氯化镁 溶液

Premium Grade
form

liquid

form

liquid

form

liquid

form

liquid

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.0002% total Nitrogen (N)

impurities

-

impurities

DNAse, none detected, NICKase, none detected, Protease, none detected, RNAse, none detected

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.0002% total nitrogen (N)

pH

5.0-7.5 (25 °C, 1 M in H2O)

pH

-

pH

-

pH

3.0-5.0 (25 °C)

solubility

water: soluble

solubility

-

solubility

water: soluble

solubility

-

density

1.08 g/mL at 20 °C

density

-

density

1.080 g/cm3

density

-

一般描述

氯化镁广泛应用于聚合酶链反应(PCR)缓冲液中提供镁离子。当使用总基因组 DNA、合成长产物或在多重反应中时,Mg2+ 的浓度是影响 PCR 的效率和特异性的最关键参数。Mg2+ 浓度的升高提高了引物模板双链体的稳定性和扩增效率。

应用

氯化镁溶液已可用于逆转录酶PFV RT的反应混合物中。它已可用于制备人工脑脊液(ACSF)。
氯化镁溶液已被用作细胞核分离缓冲液的成分,用于原位固定和从库普弗细胞(或其他肝细胞)分离细胞核。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves


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