推荐产品
品質等級
形狀
solid
分子量
15-600 kDa
分析物化學類別
amino acids, peptides, proteins
技術
gel permeation chromatography (GPC): suitable
應用
clinical
food and beverages
pharmaceutical
形式
neat
儲存溫度
−20°C
一般說明
蛋白标准品混合物是校准用标准品,用于测试和监测尺寸排阻色谱(SEC)柱的性能。它是分子量从 15 kDa 到 600 kDa 的标记物的冻干混合物。
组分
甲状腺球蛋白牛 MW ~ 670 000 Da
牛血液中的 γ-球蛋白 MW ~ 150 000 Da
卵白蛋白 MW~ 44 300 Da
核糖核酸酶 A I-A 型 MW ~ 13 700 Da
对氨基苯甲酸 (pABA) MW ~ 137 Da
组分
甲状腺球蛋白牛 MW ~ 670 000 Da
牛血液中的 γ-球蛋白 MW ~ 150 000 Da
卵白蛋白 MW~ 44 300 Da
核糖核酸酶 A I-A 型 MW ~ 13 700 Da
对氨基苯甲酸 (pABA) MW ~ 137 Da
應用
本分析标准用于以下方面:
- 通过不同流速、硅胶颗粒大小和柱孔大小评价粒径排除色谱(SEC)法分离完整蛋白的选择性和分离效率
- 通过粒径排除色谱-高效液相色谱-二极管阵列检测(SEC-HPLC-DAD)同时测定寡聚化和硝化蛋白
- 以牛血清白蛋白(BSA)作为模型蛋白,经 O3 和 NO2 诱导氧化、硝化和寡聚,通过尺寸排除层析法对蛋白质进行分子量分离
- 通过粒径排除色谱(SEC)法估算两种重组蛋白 TNF 荧光传感器(BTN-Kat)和荧光传感器抑制剂(ITN-Kat)的分子量,评估其在体外结合和中和肿瘤坏死因子(TNF)的能力,并进一步作为非侵入性分析的成像标记
相關產品
产品编号
说明
价格
訊號詞
Danger
危險分類
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
其他客户在看
Marc D Zack et al.
Scientific reports, 7(1), 11112-11112 (2017-09-13)
In this work, we characterized 2 novel insecticidal proteins; Vip3Ab1 and Vip3Bc1. These proteins display unique insecticidal spectra and have differential rates of processing by lepidopteran digestive enzymes. Furthermore, we have found that both proteins exist as tetramers in their
Anastasiya Lavell et al.
The Plant journal : for cell and molecular biology, 108(5), 1332-1345 (2021-09-29)
Rhomboid-like proteins are intramembrane proteases with a variety of regulatory roles in cells. Though many rhomboid-like proteins are predicted in plants, their detailed molecular mechanisms or cellular functions are not yet known. Of the 13 predicted rhomboids in Arabidopsis thaliana
Simultaneous determination of nitrate and oligomerized proteins by size exclusion high-performance liquid chromatography coupled to photodiode array detection.
Liu F, et al.
Journal of Chromatography A, 1495, 76-82 (2017)
Rodrigo Melgosa et al.
Foods (Basel, Switzerland), 10(6) (2021-06-03)
The valorization of Atlantic cod (Gadus morhua) frames from a filleting industry was investigated using subcritical water extraction and hydrolysis (SBW) at different temperatures (90, 140, 190 and 250 °C) and 100 bar to obtain extracts rich in proteins, peptides
Chunjun Yan et al.
Food research international (Ottawa, Ont.), 141, 110163-110163 (2021-03-02)
This study investigated the effects of walnut phenolics and extraction methods on the composition and structural properties of walnut protein isolates (WPIs). Fluorescence quenching experiments showed that walnut phenolics could bind to walnut globulins, albumins, and glutelins with apparent affinity
商品
Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.
体积排阻色谱(SEC)色谱柱和现成的标准品有助于方法开发,并提高蛋白质SEC方法的耐用性。
Characterize mAb monomers, aggregates, and fragments using SEC-UV workflow with Zenix® and Zenix®-C SEC columns.
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持