Merck
All Photos(1)

93321

Sigma-Aldrich

TRIS 甘氨酸缓冲液 溶液

BioUltra, 10× concentrate

Synonym(s):
印迹缓冲剂 溶液

产品线

BioUltra

质量水平

100

形式

liquid

质量

10× concentrate

组成

glycine, 1.92 M
TRIS, 0.25 M

杂质

insoluble matter, passes filter test
proteases, none detected

灼烧残渣

≤0.2%

pH值(酸碱度)

8.5-8.7

痕量阴离子

sulfate (SO42-): ≤50 mg/kg

痕量阳离子

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
NH4+: ≤200 mg/kg
Na: ≤500 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

neat

紫外吸收

λ: 260 nm Amax: 0.1
λ: 280 nm Amax: 0.1

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应用

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. TRIS Glycine buffer solution, 10X concentrate is diluted to a working concentration of 1X and pH adjusted as required. TRIS Glycine buffer (TGB) is frequently used in gel electrophoresis and ion-exchange chromatography applications.

其他说明

从聚丙烯酰胺凝胶到硝化纤维片的蛋白质电泳转移:步骤和一些应用

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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