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ATG001

Raji CD19 Antigen Panel Cell Lines

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此商品	ATG004	HLA001	119M-1
Sigma-Aldrich ATG001 Raji CD19 Antigen Panel Cell Lines	Sigma-Aldrich ATG004 A375 NY−ESO−1 Antigen Panel Cell Lines	Sigma-Aldrich HLA001 Raji HLA Panel Cell Lines	Sigma-Aldrich 119M-1 CD19 (MRQ-36) Mouse Monoclonal Antibody
growth mode suspension	growth mode adherent	growth mode suspension	growth mode -
morphology lymphoblast	morphology epithelial	morphology lymphoblast	morphology -
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in wet ice
storage temp. −196°C	storage temp. −196°C	storage temp. −196°C	storage temp. 2-8°C

一般描述

This product is a panel of three (3) genetically modified cell lines involving the CD19 gene. The panel consists of a CD19 knockout cell line (CD19^-/-) as well as a CD19^low and a CD19^high expression cell line. The CD19^-/- cell line was generated using CompoZr^® Zinc Finger Nuclease technology, and the two CD19 expression cell lines were generated using MISSION^® lentivirus. The CD19^low and CD19^high cell lines express CD19 at low and high levels relative to the CD19^-/- cell line (CD19^high > CD19^low > CD19^-/-) as measured via fluorescence-activated cell sorting (FACS). As a comparison, wild type Raji cells demonstrate CD19^medium expression as measured via FACS.

The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 85011429.

细胞系描述

Established in 1963 from Burkitt′s lymphoma in an 11 year old male. Growth is in the form of single cells without attachment and as macroscopically visible clumps containing many hundreds of cells. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. Raji is sometimes referred to as a ′non-producer′; the EBV genome carries deletions attributed to preventing the formation of virus particles. Ethnicity: Black

特点和优势

The Raji CD19 antigen panel cell lines feature CD19 expressed at varying levels (KO, low, and high). These cell lines enable researchers to screen and evaluate the binding and potency of CD19-targeted immunotherapies in a biologically-relevant cellular research model.

组分

ATG001A-1VL: Raji CD19 Knockout
ATG001B-1VL: Raji CD19 Low
ATG001C-1VL: Raji CD19 High

质量

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, and short terminal repeat (STR) analysis for cell line identification.

法律信息

MISSION Use Licenses Agreement

ZFN Use Licenses Agreement

CompoZr is a registered trademark of Sigma-Aldrich Co. LLC

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

储存分类代码

6.2 - Infectious substances

WGK

WGK 2

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Immuno Oncology Research Tools and Solutions

Whether you need screening tools for CAR/TCR therapies and mAB development, target cells for efficacy testing, or potency assays for clinical development, we provide functional biologically-relevant solutions to help you save time, accelerate your immuno-oncology research, and simplify your workflow.

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Raji CD19 Antigen Panel Cell Lines

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