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Merck
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文件

B6529

Sigma-Aldrich

亮蓝R染色液

ethanol solution

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别名:
酸性蓝83, Coomassie 亮蓝R, 亮吲哚蓝蛋白 6B, 酸性蓝 83
经验公式（希尔记法）:
C45H44N3NaO7S2
CAS号:
6104-59-2
分子量:
825.97
顏色索引編號:
42660
Beilstein:
5718025
MDL號碼:
MFCD00041762
分類程式碼代碼:
12171500
PubChem物質ID:
24891914
NACRES:
NA.47

形狀

liquid

品質等級

200

顏色

dark blue

適合性

suitable for (for immunoelectrophoresis protein staining)

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

SMILES 字串

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

InChI 密鑰

NKLPQNGYXWVELD-UHFFFAOYSA-M

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應用

亮蓝R染色溶液专门设计用于在免疫电泳和Ouchterlony凝胶之后对琼脂糖凝胶中的蛋白质进行染色。污渍含有乙醇和乙酸，因此在染色前无需固定凝胶。首先用水和盐水洗涤免疫电泳或Ouchterlony凝胶，以去除未沉淀的蛋白质，然后干燥。然后将凝胶浸入染色溶液中30分钟，并用10%乙酸脱色。
电泳后检测蛋白条带。

成分

0.5% (w/v) 亮蓝 R、45% (v/v) 乙醇和 10% (v/v) 乙酸。

分析報告

在免疫电泳后测试琼脂糖凝胶的适用性。

法律資訊

象形圖

FlameExclamation mark
GHS02,GHS07

訊號詞

Warning

危險聲明

H226,H315,H319

危險分類

Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2

水污染物質分類（WGK）

WGK 2

閃點（°F）

81.0 °F

閃點（°C）

27.2 °C

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

分析证书(COA)

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Wei Zhang et al.
International journal of oncology, 54(5), 1719-1733 (2019-03-14)
Ovarian cancer remains the most lethal type of cancer among all gynecological malignancies. The majority of patients are diagnosed with ovarian cancer at the late stages of the disease. Therefore, there exists an imperative need for the development of early
Reiner Westermeier
Proteomics, 6 Suppl 2, 61-64 (2006-10-13)
In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There
G Houen et al.
Electrophoresis, 18(5), 701-705 (1997-05-01)
A method for staining proteins on polyvinylidene difluoride membranes without using organic solvent is described. The method uses preblocking of the membrane with either Tween 20 or polyethylene glycol followed by staining with 0.01% Coomassie Brilliant Blue. No destaining of
Melda Altikatoglu et al.
Artificial cells, blood substitutes, and immobilization biotechnology, 39(3), 185-190 (2010-12-02)
Horseradish peroxidase (EC 1.11.1.7) was chemically modified by periodate-activated dextran. The activities of free and modified enzyme against organic-aqueous interface and some chemicals were determined. Modified HRP remained fully active in the presence of organic solvent for 4 h. However
Yong-Hak Kim et al.
Archives of biochemistry and biophysics, 494(2), 159-165 (2009-12-01)
We studied the decolorization of malachite green (MG) by the fungus Cunninghamella elegans. The mitochondrial activity for MG reduction was increased with a simultaneous increase of a 9-kDa protein, called CeCyt. The presence of cytochrome c in CeCyt protein was
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