Merck
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C2-BIOC

Sigma-Aldrich

II型胶原酶,Cls II

形式

powder

质量水平

储存温度

2-8°C

Related Categories

一般描述

该产品与Biochrom GmbH部件号C2-22和C2-28相同。由胶原酶、梭菌蛋白酶混合而成的酶,具有来自溶组织梭菌的胰蛋白酶和蛋白水解活性。该制剂显示高梭菌蛋白酶活性,其胰蛋白酶活性接近于I型。适用于肝脏,骨骼,甲状腺,心脏和唾液腺组织。比活度为每毫克粉末物质125至250 Mandl单位。
Collagenase Type II (Cls II) is one of the main tissue dissociation enzymes produced by Clostridium histolyticum. It aids to digest the smaller collagen fragments produced by Type I collagenase. Collagenases, a member of the matrix metalloproteinase (MMPs) family, digests collagen in the extracellular matrix (ECM). Collagens are triple helix structural proteins comprising three collagen polypeptides with high tensile strength.

应用

Collagenase Type II (Cls II) has been used:
  • as a component of Roswell park memorial Institute (RPMI)-1640 media to digest hepatic tissues
  • to perfuse murine heart for the isolation of epicardial stromal cells (EpiSC)
  • to digest collagenase and inject it into the intrarenal aorta abdominalis to perfuse the kidneys

包装

100 mg
1 g

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