Merck

E4884

Sigma-Aldrich

乙二胺四乙酸 二钠盐 二水合物

ACS reagent, 99.0-101.0%

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别名:
EDTA 二钠盐, EDTA-Na2, 乙二胺四乙酸Na2, 乙二胺四乙酸盐 二钠盐 二水合物, 二钠乙二胺四乙酸盐 二水合物, 依地酸钙钠
Empirical Formula (Hill Notation):
C10H14N2Na2O8 · 2H2O
CAS号:
分子量:
372.24
Beilstein:
3900609
EC 号:
PubChem化学物质编号:
NACRES:
NA.77

等级

ACS reagent

质量水平

Agency

suitable for SM 4500 - NH3

检测方案

99.0-101.0%

形式

solid

reaction suitability

reagent type: chelator

杂质

≤0.005% Insoluble matter
≤0.1% Nitrilotriacetic acid ([HOCOCH2]3N)

pH值(酸碱度)

4.0-6.0 (25 °C, 5%)

mp

248 °C (dec.) (lit.)

溶解性

H2O: 100 g/L at 20 °C

痕量阳离子

Fe: ≤0.01%
heavy metals: ≤0.005% (by ICP-OES)

application(s)

microbiology

储存温度

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

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此商品
ED2SSE164427285
Ethylenediaminetetraacetic acid disodium salt dihydrate ACS reagent, 99.0-101.0%

Sigma-Aldrich

E4884

乙二胺四乙酸 二钠盐 二水合物

Essential Grade
Ethylenediaminetetraacetic acid disodium salt dihydrate reagent grade, 98.5-101.5% (titration)

Sigma-Aldrich

ED2SS

乙二胺四乙酸 二钠盐 二水合物

Essential Grade
Ethylenediaminetetraacetic acid disodium salt dihydrate 98.5-101.5%, BioUltra

Sigma-Aldrich

E1644

乙二胺四乙酸 二钠盐 二水合物

Premium Grade
Ethylenediaminetetraacetic acid disodium salt dihydrate meets analytical specification of Ph. Eur., BP, USP, FCC, 99.0-101.0%

Sigma-Aldrich

27285

乙二胺四乙酸 二钠盐 二水合物

Essential Grade
assay

99.0-101.0%

assay

98.5-101.5% (titration)

assay

98.5-101.5%

assay

99.0-101.0%

form

solid

form

powder

form

powder

form

crystals

impurities

≤0.005% Insoluble matter, ≤0.1% Nitrilotriacetic acid ([HOCOCH2]3N)

impurities

-

impurities

≤0.005% Phosphorus (P), ≤0.05% NH3, ≤0.1% Insoluble matter

impurities

residual solvents, complies, ≤0.001% cyanide (CN), ≤0.001% heavy metals (as Pb), ≤0.1% nitrilotriacetic acid (HPLC) (HPLC)

pH

4.0-6.0 (25 °C, 5%)

pH

-

pH

4.0-6.0 (0.1 M in H2O)

pH

4-5 (20 °C, 5%)

mp

248 °C (dec.) (lit.)

mp

248 °C (dec.) (lit.)

mp

248 °C (dec.) (lit.)

mp

248 °C (dec.) (lit.)

一般描述

Ethylenediaminetetraacetic acid disodium salt dihydrate or EDTA, is a chelating agent with many applications in molecular biology. EDTA is often used in buffers as a metal ion inhibitor to maintain the stability and integrity of biological samples. EDTA binds to metal cations by forming a stable octahedral complex. Its ability to chelate metal ions makes it extremely useful for blocking metal-dependent enzymatic activities, such as DNases and proteases. Additionally, EDTA is often found in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

应用

乙二胺四乙酸二钠水合物已被用作以下物质的成分:
  • 用于制备C2C12小鼠骨骼肌成肌细全细胞裂解液的放射免疫沉淀测定缓冲液(RIPA缓冲液)
  • 用于纯化人蛋白酶体调节颗粒(RP)的裂解缓冲液和IgG树脂洗涤和平衡缓冲液
  • 用于从胎儿心脏中分离人心肌祖细胞(CMPC)的M缓冲液

Antioxidant assays (1) EDTA is also employed in molecular biology techniques, such as DNA extraction, purification, and electrophoresis, where it helps to prevent metal-mediated degradation of nucleic acids. Additionally, EDTA is utilized in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.
二价阳离子的螯合剂。 对需要二价阳离子发挥活性的酶,比如金属蛋白酶具有抑制作用。

特点和优势

American Chemical Society (ACS) grade is suitable for use in standard analytical methods

象形图

Exclamation markHealth hazard

警示用语:

Warning

危险声明

危险分类

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

靶器官

Respiratory Tract

储存分类代码

11 - Combustible Solids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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