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F9252

Sigma-Aldrich

Folin & Ciocalteu 酚试剂

suitable for determination of total protein by Lowry method, 2 N

MDL编号:
NACRES:
NA.47

形式

liquid

质量水平

200

浓度

2 N

应用

hematology: suitable
histology: suitable

pH值(酸碱度)

<0.5 (20 °C)

密度

1.240 g/cm3 at 20 °C

适用性

suitable for determination of total protein by Lowry method

特色行业

Diagnostic Assay Manufacturing

储存温度

room temp

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一般描述

Folin & Ciocalteu酚试剂最常用于Lowry法测定蛋白浓度。它还用于总酚类的定量。在该方法中,蛋白质用改进的缩二脲试剂(用酒石酸钠钾稳定的碱性铜溶液
)中的铜(II)进行预处理。酚试剂的加入可产生在550-750 nm间提高吸光值的色原。通常,在尖峰(750 nm)或肩峰(660 nm)处的吸光度用于定量1-100 mg/ml之间的蛋白浓度,而550nm处的吸光度用于定量更高的蛋白浓度。
在没有铜的情况下,颜色强度主要由蛋白中的酪氨酸和色氨酸含量决定,并在较小程度上由半胱氨酸和组氨酸决定。铜(II)对由酪氨酸、色氨酸或组氨酸而形成的颜色没有影响,但会由于半胱氨酸而减少颜色形成。
许多对原始检测流程的修改已得到发表,包括用于增强显色、测定不溶性蛋白含量以及过程的自动化。多种物质包括许多缓冲剂、螯合剂、去污剂和环状有机化合物都可对Lowry蛋白检测产生干扰。
Folin & Ciocalteu酚试剂可在色谱过程中用作喷雾剂。

应用

Folin & Ciocalteu酚试剂已用于样品中总酚量的预测。

包装

1 L in glass bottle
100, 500 mL in glass bottle

其他说明

Folin & Ciocalteu酚试剂中不含酚。反而,试剂可与酚类和非酚类还原性物质反应形成可通过分光光度法检测的色原。

联系

该产品也还作为试剂盒的一部分。

象形图

Corrosion

警示用语:

Danger

危险声明

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK Germany

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

  1. What concentrations of proteins does Product F9252, Folin & Ciocalteu's phenol reagent, detect?

    Generally, Folin & Ciocalteu's phenol reagent is used to determine protein concentrations between 1 to 100 μg/mL. However, the reagent exhibits a linear response to protein concentration up to 1000 μg/mL when used according to the instructions for kit Product No. TP0200.

  2. Can Product F9252, Folin & Ciocalteu's phenol reagent, be used to detect any protein?

    If the protein has an abnormally low tyrosine content, this method would not be suitable.

  3. What should Product F9252, Folin & Ciocalteu's phenol reagent, look like?

    The Folin & Ciocalteu's phenol reagent should be a clear yellow solution.  If the solution turns green, it may not be suitable and it should not be used.

  4. Does Product F9252, Folin & Ciocalteu's phenol reagent, contain phenol?

    The Folin & Ciocalteu's phenol reagent does not contain phenol but reacts with phenol and non-phenolic reducing substances to form chromogens that can be detected spectrophotometrically.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

H J Fryer et al.
Analytical biochemistry, 153(2), 262-266 (1986-03-01)
The method of protein determination reported by Lowry et al. (1951, J. Biol. Chem. 193, 265-275) has been adapted for use with 96-well microtiter plates and an automatic microplate spectrophotometer. The spectrophotometer has been interfaced with a computer which plots...
Nutritional evaluation of some subtropical red and green seaweeds Part II. In vitro protein digestibility and amino acid profiles of protein concentrates.
Wong KH and Cheung PCK
Food Chemistry, 72, 11-17 (2001)
Picheswara Rao Polu et al.
BMC complementary and alternative medicine, 17(1), 457-457 (2017-09-13)
Tinospora cordifolia (Guduchi or Amrita) is an important drug of Ayurvedic System of Medicine and found mention in various classical texts for the treatment of diseases such as jaundice, fever, diabetes, cancer and skin disease etc. In view of its...
Lowry's method of protein estimation: some more insights.
S Sengupta et al.
The Journal of pharmacy and pharmacology, 45(1), 80-80 (1993-01-01)
Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent.
Ainsworth EA and Gillespie KM
Nature Protocols, 2, 875-875 (2007)

技术文章

Enzymatic Assay of Proteinase K with Hemoglobin Substrate

Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).

实验方案

Protein Determination Modified Lowry Method

To standardize a procedure for the determination of protein by modified Lowry.

Plant-Based PCR Inhibitors During DNA Extraction and PCR Master Mix Performance

Evaluating the effects of PCR inhibitors and PCR enzyme selection using plant genomic DNA, GenElute™-E Single Spin Plant Extraction Kit, KOD-One™ PCR Master Mix and competitor high fidelity PCR master mixes.

Universal Protease Activity Assay: Casein as a Substrate

Proteases break peptide bonds. In the lab, it is often necessary to measure and/or compare the activity of proteases. Sigma's non-specific protease activity assay may be used as a standardized procedure to determine the activity of proteases, which is what we do during our quality control procedures.

Enzymatic Assay of Proteinase K (EC 3.4.21.64)

Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).

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相关内容

Tools for Protein Quantitation

A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.

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