Merck

F9252

Sigma-Aldrich

Folin & Ciocalteu 酚试剂

suitable for determination of total protein by Lowry method, 2 N

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MDL编号:
NACRES:
NA.47

形式

liquid

质量水平

浓度

2 N

颜色

clear yellow

pH值(酸碱度)

<0.5 (20 °C)

密度

1.240 g/cm3 at 20 °C

适用性

suitable for determination of total protein by Lowry method

application(s)

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

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此商品
TP0200M194227813
vibrant-m

F9252

Folin & Ciocalteu 酚试剂

vibrant-m

M1942

钼蓝喷雾试剂

vibrant-m

27813

考马斯蛋白检测试剂

concentration

2 N

concentration

-

concentration

-

concentration

-

color

clear yellow

color

-

color

-

color

-

pH

<0.5 (20 °C)

pH

-

pH

-

pH

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

room temp

storage temp.

2-8°C

storage temp.

room temp

storage temp.

2-8°C

一般描述

Folin & Ciocalteu酚试剂最常用于Lowry法测定蛋白浓度。它还用于总酚类的定量。在该方法中,蛋白质用改进的缩二脲试剂(用酒石酸钠钾稳定的碱性铜溶液
)中的铜(II)进行预处理。酚试剂的加入可产生在550-750 nm间提高吸光值的色原。通常,在尖峰(750 nm)或肩峰(660 nm)处的吸光度用于定量1-100 mg/ml之间的蛋白浓度,而550nm处的吸光度用于定量更高的蛋白浓度。
在没有铜的情况下,颜色强度主要由蛋白中的酪氨酸和色氨酸含量决定,并在较小程度上由半胱氨酸和组氨酸决定。铜(II)对由酪氨酸、色氨酸或组氨酸而形成的颜色没有影响,但会由于半胱氨酸而减少颜色形成。
许多对原始检测流程的修改已得到发表,包括用于增强显色、测定不溶性蛋白含量以及过程的自动化。多种物质包括许多缓冲剂、螯合剂、去污剂和环状有机化合物都可对Lowry蛋白检测产生干扰。
Folin & Ciocalteu酚试剂可在色谱过程中用作喷雾剂。

应用

Folin & Ciocalteu酚试剂已用于样品中总酚量的预测。

其他说明

Folin & Ciocalteu酚试剂中不含酚。反而,试剂可与酚类和非酚类还原性物质反应形成可通过分光光度法检测的色原。

联系

该产品也还作为试剂盒的一部分。

象形图

Corrosion

警示用语:

Danger

危险声明

危险分类

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1

储存分类代码

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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其他客户在看

Lowry's method of protein estimation: some more insights.
S Sengupta et al.
The Journal of pharmacy and pharmacology, 45(1), 80-80 (1993-01-01)
Nutritional evaluation of some subtropical red and green seaweeds Part II. In vitro protein digestibility and amino acid profiles of protein concentrates.
Wong KH and Cheung PCK
Food Chemistry, 72, 11-17 (2001)
Picheswara Rao Polu et al.
BMC complementary and alternative medicine, 17(1), 457-457 (2017-09-13)
Tinospora cordifolia (Guduchi or Amrita) is an important drug of Ayurvedic System of Medicine and found mention in various classical texts for the treatment of diseases such as jaundice, fever, diabetes, cancer and skin disease etc. In view of its
Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent.
Ainsworth EA and Gillespie KM
Nature Protocols, 2, 875-875 (2007)
Han-Shin Kim et al.
Scientific reports, 6, 25318-25318 (2016-05-05)
Biofilm formation on biotic or abiotic surfaces has unwanted consequences in medical, clinical, and industrial settings. Treatments with antibiotics or biocides are often ineffective in eradicating biofilms. Promising alternatives to conventional agents are biofilm-inhibiting compounds regulating biofilm development without toxicity

实验方案

To standardize a procedure for the determination of protein by modified Lowry.

Evaluating the effects of PCR inhibitors and PCR enzyme selection using plant genomic DNA, GenElute™-E Single Spin Plant Extraction Kit, KOD-One™ PCR Master Mix and competitor high fidelity PCR master mixes.

Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).

相关内容

A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.

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