• HLA002
All Photos(1)



Jurkat HLA Panel Cell Lines

CAR-T, Checkpoint Inhibitors, Immuno-oncology, Immunotherapy, Oncology


human (T Lymphocyte (blood))




dry ice




The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 88042803.

This product is a panel of eleven (11) genetically modified cell lines targeting MHC Class I HLA molecules in a Jurkat T lymphocyte parental cell line background.  The full HLA panel consists of a Beta-2 microglobulin (β2M) knockout cell line and an additional ten (10) monoallelic HLA expression cell lines.  

CompoZr® Zinc Finger Nuclease (ZFN) technology was used to generate the β2M knockout cell line, which does not express endogenous cell surface MHC Class I HLA molecules (HLA-A or HLA-B) as measured by fluorescence-activated cell sorting (FACS).  

MISSION® lentivirus was used to generate the ten (10)  monoallelic HLA cell lines, which express individual HLA-A or HLA-B subtypes on the cell surface via a β2M:HLA fusion protein as described in Nature Biotechnology 35, 765-772, (2017) and as demonstrated via FACS analysis.  


Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.


Unlike wild type Jurkat cells, which express multiple HLA subtypes, the Jurkat HLA panel cell lines express individual MHC Class I HLA subtypes. These monoallelic HLA cell lines can be used to study peptide binding and presentation in the context of individual HLA subtypes. The panel can also be used to develop cell-based assays to evaluate candidate cancer immunotherapies and identify tumor-specific neoantigens.


HLA002A-1VL: Jurkat B2M KO Cells
HLA002B-1VL: Jurkat HLA-A*02:01 Cells
HLA002C-1VL: Jurkat HLA-A*01:01 Cells
HLA002D-1VL: Jurkat HLA-A*03:01 Cells
HLA002E-1VL: Jurkat HLA-A*11:01 Cells
HLA002F-1VL: Jurkat HLA-A*24:02 Cells
HLA002G-1VL: Jurkat HLA-B*15:10 Cells
HLA002H-1VL: Jurkat HLA-B*07:02 Cells
HLA002I-1VL: Jurkat HLA-B*08:01 Cells
HLA002J-1VL: Jurkat HLA-B*35:01 Cells
HLA002K-1VL: Jurkat HLA-B*40:01 Cells


Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, and short terminal repeat (STR) analysis for cell line identification.


CompoZr is a registered trademark of Sigma-Aldrich Co. LLC
MISSION is a registered trademark of Sigma-Aldrich Co. LLC


10 - Combustible liquids



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).


Product Number
Pack Size/Quantity

Additional examples:





(enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service