Merck
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I6758

Sigma-Aldrich

IPTG

≥99% (TLC), ≤0.1% Dioxane

Synonym(s):
异丙 β-D-1-硫代半乳糖苷, IPTG, 异丙基 β-D-硫代半乳糖苷
Empirical Formula (Hill Notation):
C9H18O5S
CAS Number:
Molecular Weight:
238.30
Beilstein:
4631
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.31

生物来源

synthetic

质量水平

300

描述

non-ionic

测定

≥99% (TLC)

形式

powder

technique(s)

thin layer chromatography (TLC): suitable

杂质

≤0.1% Dioxane

储存温度

2-8°C

SMILES string

CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

InChI key

BPHPUYQFMNQIOC-NXRLNHOXSA-N

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包装

1, 5, 10 g in glass bottle

应用

IPTG 常用于需要诱导 β-半乳糖苷酶活性的克隆实验。它常与 X-Gal 或 Bluo-Gal 结合使用,用于重组细菌菌落的蓝白筛选,这些菌落可以诱导 lac 操纵子在大肠杆菌中的表达。IPTG 与 lacI 阻遏蛋白结合并改变其构象而发挥作用,防止 β-半乳糖苷酶编码基因 lacZ 的抑制。

其他说明

非代谢性半乳糖类似物。

重悬

将 IPTG 溶于水,然后经无菌过滤,配制成储存液 (0.1M)。指示板中 IPTG 最终浓度应为 0.2mM。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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