Merck

P2308

Sigma-Aldrich

蛋白酶 K 来源于林伯氏白色念球菌

lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

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别名:
肽链内切酶
CAS号:
EC 号:
MDL编号:
eCl@ss:
32160410
NACRES:
NA.54

等级

for molecular biology

质量水平

产品线

BioUltra

形式

lyophilized powder

specific activity

≥30 units/mg protein

分子量

28.93 kDa

组成

Protein, ≥90% biuret

杂质

≤1 ppm DNA

异质活性

DNAse, Nickase and RNAse, none detected

运输

wet ice

储存温度

−20°C

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此商品
P6556SRE0047SRE0005
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

buffered aqueous glycerol solution

specific activity

≥30 units/mg protein

specific activity

≥30 units/mg protein

specific activity

≥30 units/mg protein

specific activity

-

mol wt

28.93 kDa

mol wt

28.93 kDa

mol wt

28.93 kDa

mol wt

28.93 kDa

impurities

≤1 ppm DNA

impurities

-

impurities

≤1 ppm DNA

impurities

≤500 pg/mg DNA (PicoGreen® assay)

foreign activity

DNAse, Nickase and RNAse, none detected

foreign activity

Dnase ≤30 Kunitz units/mg solid, RNase ≤0.003 Kunitz units/mg solid

foreign activity

DNAse, Nickase and RNAse, none detected

foreign activity

DNAse, RNAse, exonuclease, endonuclease, and nickase, none detected

应用

在分离DNA和RNA的过程中,用于核酸酶的蛋白水解失活。
用于去除内毒素结合的阳离子蛋白,如溶菌酶和核糖核酸酶A。
据报道,其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理,暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。

生化/生理作用

蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族,具有活性位点催化三联体(Asp39-His69-Ser224)。它在一系列广泛的环境中均稳定:pH,缓冲盐,去污剂(SDS)以及温度。 在0.1-0.5% SDS存在的情况下,蛋白酶K可保持活性,在不损害分离DNA完整性的前提下,可以消化DNA制剂中的多种蛋白质和核酸酶。

单位定义

在pH 7.5,37 °C 条件下,一单位的酶每分钟将会尿素变性的血红蛋白以产生与1.0 μ摩尔 (181 μg) 酪氨酸等同的颜色(Folin-Ciocalteu试剂提供的颜色)。

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Mamoru Oshiki et al.
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Detection and genotyping of pathogenic RNA viruses in human and environmental samples are useful for monitoring the circulation and prevalence of these pathogens, whereas a conventional PCR assay followed by Sanger sequencing is time-consuming and laborious. The present study aimed
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实验方案

GenElute™ Blood Genomic DNA Kit Protocol

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