Merck
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STS0200

Sigma-Aldrich

TWEEN ® 20

greener alternative

viscous liquid

Synonym(s):
聚氧乙烯山梨糖醇酐单月桂酸酯, 聚氧乙烯脱水山梨醇单月桂酸酯
CAS Number:
MDL编号:

描述

non-ionic

质量水平

200

测定

≥40.0% (GC)

形式

viscous liquid

分子量

~1228

组成

lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)

环保替代产品特性

Use of Renewable Feedstocks
Design for Degradation
Learn more about the Principles of Green Chemistry.

杂质

≤3.0% water

折射率

n20/D 1.468 (lit.)

CMC

0.06 mM (20-25°C)

酸值

≤2.2 mg KOH/g

羟基值

96‑108 mg KOH/g

转变温度

cloud point 76 °C

密度

1.095 g/mL at 25 °C (lit.)

HLB

16.7

环保替代产品分类

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

InChI key

HMFKFHLTUCJZJO-UHFFFAOYSA-N

一般描述

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Green Chemistry. This product is a biobased surfactant and is aligned with the 7th principle of Green Chemistry "Use of Renewable Feedstocks" and the 10th principle "Design for Degradation".

TWEEN 20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.[1]

应用

TWEEN 20 has been used as a component of:
  • washing buffer and blocking buffer in Western blotting[2]
  • blocking buffer in immunohistochemistry[3]
  • washing buffer in ELISA (enzyme linked immunosorbent assay)[4][5]
  • reaction mixture in PCR (polymerase chain reaction)[6]
TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
非离子洗涤剂

特点和优势

  • 100 % Renewable
  • 100 % Bio-based
  • Certified to the USDA BioPreferred Program
  • Lower carbon footprint than petrochemical-based versions

制备说明

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

法律信息

TWEEN is a registered trademark of Croda International PLC

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(F)

527.0 °F - Pensky-Martens closed cup

闪点(C)

275 °C - Pensky-Martens closed cup

Certificate of Analysis

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Certificate of Origin

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More Documents

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