Merck
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X100

Sigma-Aldrich

Triton X-100

laboratory grade

Synonym(s):
4-(1,1,3,3-四甲基丁基)苯基-聚乙二醇, 叔辛基苯氧基聚乙烯乙氧基乙醇, 聚乙二醇叔辛基苯基醚
Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
Beilstein:
2315025
MDL编号:
PubChem化学物质编号:
NACRES:
NA.21

质量水平

200

等级

laboratory grade

描述

non-ionic

形式

liquid

分子量

micellar avg mol wt 80,000
average mol wt 625

聚集数

100-155

CMC

0.2-0.9 mM (20-25°C)

转变温度

cloud point 65 °C
pour point ~7 °C

溶解性

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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一般描述

Triton X-100具有溶血作用,可用于DNA提取。
Triton X-100是一种常用的非离子型的表面活性剂和乳化剂,经常用于生化应用以溶解蛋白质。它被视为一种不变性的相对温和的洗涤剂,并且在许多参考文献中作为一种常规被添加的试剂而被报道。它用于裂解细胞以提取蛋白和细胞器。还可通透活细胞膜用于转染。

应用

Triton-X已用于:
  • 细胞通透化处理用于免疫荧光染色
  • western blot分析中裂解缓冲液中的一种成分
  • 制备免疫金标记的细胞切片用于电子显微镜的Tris盐缓冲液中的一种成分

包装

5 mL in glass bottle
1 gal in poly bottle
5 gal in poly drum
1 L in poly bottle
100, 500 mL in poly bottle

生化/生理作用

广泛使用的非离子表面活性剂,用于在温和的非变性条件下进行膜恢复。

法律信息

Tri-Chek is a trademark of Micro Essential Laboratory, Inc.
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

483.8 °F

闪点(C)

251 °C

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter, type N95 (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Articles

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Protocols

Enzymatic Assay of Cholesterol Oxidase

This procedure applies to products that have a specification for the enzymatic activity of cholesterol oxidase. This assay is NOT to be used to assay Cholesterol Oxidase from Schizophyllum commune (Discontinued Product Number C7274) and from Brevibacterium sp. (Discontinued Product Number C8153).

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