Merck

04511

Supelco

活/死细胞双染色试剂盒

suitable for fluorescence

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别名:
用于活/死细胞染色
NACRES:
NA.32

适用性

suitable for fluorescence

质量水平

储存温度

−20°C

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BR472200SCT083BR474305
Supelco

Supelco

04511

活/死细胞双染色试剂盒

BRAND® glass staining trough with lid soda-lime glass

BR472200

BRAND®有盖玻璃染色槽

BRAND® transparent plastic staining tray Holds 20 x slides, for use with transparent plastic staining trough

BR474305

BRAND®透明塑料染色盘

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

storage temp.

-

Quality Level

100

Quality Level

-

Quality Level

100

Quality Level

-

应用

该活/死细胞复染试剂盒可同时对活细胞和死细胞进行荧光染色。该试剂盒包含钙黄绿素-AM和碘化丙啶(PI)两种溶液,分别用于染色活细胞和死细胞。其中钙黄绿素-AM,即钙黄绿素的乙酰羟甲基酯,具有高度亲脂性和细胞膜透过性。尽管钙黄绿素-AM自身并非荧光分子,但活细胞中的酯酶可以催化钙黄绿素-AM生成钙黄绿素,从而发出强烈的绿色荧光(λex 490 nm, λem 515 nm). 因此钙黄绿素-AM只能染色活细胞。而核染色染料PI不能透过活细胞的细胞膜。但可以透过死细胞膜的变性区域来到达细胞核,并与细胞中的DNA双螺旋结构结合,从而发出红色荧光(λex 535 nm, λem 617 nm)。钙黄绿素和PI-DNA都能被490nm波长的光激发,因此可以采用荧光显微镜同时监测活细胞和死细胞。使用λex激发光时只能观察到死亡细胞。

仅试剂盒组分

产品编号
说明

  • Solution A (Calcein AM solution) 4 × 50

  • Solution B (propidium iodide solution) 300 μL

相关产品

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

185.0 °F - closed cup

闪点(°C)

85 °C - closed cup


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