Merck

106-05A

Sigma-Aldrich

人皮肤成纤维细胞:HDF,成人

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别名:
HDF cells
NACRES:
NA.81

生物来源

human neonatal foreskin or adult skin (normal)

质量水平

包装

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

生长模式

Adherent

核型

2n = 46

形态学

Fibroblast

technique(s)

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

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此商品
602-05A306-05FR106-05A
packaging

pkg of 500,000 cells

packaging

pkg of 500,000 cells

packaging

pkg of 500,000 cells

packaging

pkg of 500,000 cells

growth mode

Adherent

growth mode

Adherent

growth mode

Adherent

growth mode

Adherent

karyotype

2n = 46

karyotype

-

karyotype

2n = 46

karyotype

-

morphology

Fibroblast

morphology

mesenchymal

morphology

fibroblast

morphology

Fibroblast

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

一般描述

特定批次的订单不能网上下单。请联系您当地的销售代表了解更多详情。

真皮成纤维细胞负责产生形成皮肤结缔组织的细胞外基质,并在伤口愈合过程中发挥关键作用。HDF为多方面研究细胞生理学提供了一个极好的模型系统,并已在许多研究论文中使用,特别是那些与皮肤生物学和重编程/诱导的多能性研究相关的论文。通过使用当前经典的Oct3/4、Sox2、Klf4和c-Myc的 “Yamanaka混合物” ,可以利用HDF与人类滑膜细胞仪器构建诱导多能干细胞(iPSC),Shinya Yamanaka博士因该发现于2012年获得诺贝尔奖(Takanashi, 2007; 2009),此外,还可用于表征iPSC的一般性质、miRNA表达谱和分化能力(Hayashi,2010; Ohta,2011; Koyanagi-Aoi,2013; Noguchi,20130; Razak,2013; Sakurai,2013; Tanaka,2013; Yanagida,2013)

细胞系来源

皮肤

应用

损伤恢复,结缔组织研究,层粘连蛋白和纤连蛋白生成,细胞外基质,组织结构,伤口愈合,成纤维细胞生长因子反应

组分

含10% FBS & 10% DMSO的成纤维细胞基础培养基。

制备说明

  • 原代培养, >500,000个细胞在含10% FBS & 10% DMSO的成纤维细胞基础培养基中。
  • 可培养至少16次倍增。

培养基

传代培养常规

请参阅HDF培养方案

免责声明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

自备

产品编号
说明
价格

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Michele Galluccio et al.
Molecular biology reports, 47(9), 7283-7289 (2020-08-11)
It is well established that Escherichia coli represents a powerful tool for the over-expression of human proteins for structure/function studies. In many cases, such as for membrane transporters, the bacterial toxicity or the aggregation of the target protein hamper the
Manthan Patel et al.
BMC genetics, 21(1), 84-84 (2020-07-31)
The human CGGBP1 binds to GC-rich regions and interspersed repeats, maintains homeostasis of stochastic cytosine methylation and determines DNA-binding of CTCF. Interdependence between regulation of cytosine methylation and CTCF occupancy by CGGBP1 remains unknown. By analyzing methylated DNA-sequencing data obtained
Lucie Peterková et al.
Tissue & cell, 58, 121-129 (2019-05-28)
Surface modification is an important step in making a synthetic polymer cytocompatible. We have previously reported improved cytocompatibility of immortalized human keratinocytes (HaCaT) with the otherwise bioinert fluorinated ethylene propylene (FEP) upon treatment with argon plasma discharge. In this article

实验方案

本方案内容:储存——培养的准备工作——培养HDF——传代培养HDF

This protocol contains: Storage - Preparation for Culturing - Culturing HDF - Subculturing HDF

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