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10771

Sigma-Aldrich

Histopaque®-1077

sterile-filtered, density: 1.077 g/mL

NACRES:
NA.75

无菌性

sterile-filtered

质量水平

400

保质期

Expiry date on the label.

应用

hematology: suitable
histology: suitable

杂质

endotoxin, tested

密度

1.077 g/mL

储存温度

2-8°C

一般描述

Histopaque-1077是一种无菌、经内毒素检测的聚蔗糖及泛影钠溶液,并调整密度至1.077g/mL。这种即用型的培养基可有助于从小体积的全血中进行活性淋巴细胞和其他单核细胞的快速恢复。

应用

Histopaque-1077已用于
  • 外周血单核细胞(PBMC)以及骨髓单核细胞(BMMC)的分离
  • 通过离心进行单核细胞的纯化
  • 人类淋巴细胞的分离
  • 从全血中进行乳腺癌循环肿瘤细胞(CTC)的分离

法律信息

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

象形图

Health hazard

警示用语:

Danger

危险声明

Hazard Classifications

Carc. 1B - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

  1. Is Histopaque®, Product 10771, the same as Ficoll® solution, Product F5415?

    Ficoll-Hypaque is a mixture of Ficoll (polysucrose) and Hypaque (sodium diatrizoate).  It does not specify a density.  Product No. 10771 is a mixture of polysucrose and sodium diatrizoate at a density of 1.077 g/ml.

  2. Should I use Histopaque® 10771 or 10831 for isolation of lymphocytes?

    The different product numbers reflect the density of the solution.  Product No. 10771 is to be used to isolate human mononuclear cells.  Product No. 10831 is to be used for the isolation of mononuclear cells from mice, rats and other mammals.

  3. What is the difference between Histopaque® Product No. 10771 and H8889?

    Product 10771, Histopaque®-1077, is not cell culture tested and Product H8889, Histopaque®-1077 Hybri-Max, is cell culture tested.Both Products are sterile, endotoxin tested and intended for Research Use Only. 

  4. What is the osmolarity of the Product 10771, Histopaque®-1077 solution? 

    The osmolarity is a property that is tested for each lot of material; the range of acceptable values is 280.0 - 300.0 mOs/kg.  The lot specific value is given on the Certificate of Analysis.  Certificates of Analysis can be viewed on our website by entering the lot number on our Advanced Search Page.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Evaluation of respiration with clark type electrode in isolated mitochondria and permeabilized animal cells.
Silva AM and Oliveira PJ
Methods in Molecular Biology, 810, 7-24 (2012)
Erin E Thacker et al.
Vaccine, 27(50), 7116-7124 (2009-09-30)
Targeting viral vectors encoding tumor-associated antigens to dendritic cells (DCs) in vivo is likely to enhance the effectiveness of immunotherapeutic cancer vaccines. We have previously shown that genetic modification of adenovirus (Ad) 5 to incorporate CD40 ligand (CD40L) rather than...
Maria Björkqvist et al.
The Journal of experimental medicine, 205(8), 1869-1877 (2008-07-16)
Huntington's disease (HD) is an inherited neurodegenerative disorder characterized by both neurological and systemic abnormalities. We examined the peripheral immune system and found widespread evidence of innate immune activation detectable in plasma throughout the course of HD. Interleukin 6 levels...
Ana C Leal et al.
Scientific reports, 7(1), 6438-6438 (2017-07-27)
Cancer patients are at an increased risk of developing thromboembolic complications. Several mechanisms have been proposed to explain cancer-associated thrombosis including the release of tumor-derived extracellular vesicles and the activation of host vascular cells. It was proposed that neutrophil extracellular...
Coronary intraplaque hemorrhage evokes a novel atheroprotective macrophage phenotype.
Boyle JJ et al
The American Journal of Pathology, 174(3), 1097-1108 (2009)

技术文章

Comet Assay

The Comet Assay, also called single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for quantifying and analyzing DNA damage in individual cells.

实验方案

Histopaque® Density Gradient Media Troubleshooting Guide

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

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