Merck

32670

Sigma-Aldrich

4′,6-二脒基-2-苯基吲哚 二盐酸盐

BioReagent, suitable for fluorescence, ≥95.0% (HPLC)

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别名:
2-(4-氨基苯基)-6-吲哚甲酰胺 二盐酸盐, DAPI 二盐酸盐
经验公式(希尔记法):
C16H15N5 · 2HCl
CAS号:
分子量:
350.25
Beilstein:
4894417
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.32

产品线

BioReagent

质量水平

检测方案

≥95.0% (HPLC)

形式

powder

溶解性

DMF: soluble
H2O: soluble

荧光

λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA

适用性

suitable for fluorescence

储存温度

room temp

SMILES字符串

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

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此商品
MBD0015D841710236276001
DAPI成品溶液 For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL

MBD0015

DAPI成品溶液

-
4′,6-二脒基-2-苯基吲哚 二盐酸盐 powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC), suitable for fluorescence

D8417

4′,6-二脒基-2-苯基吲哚 二盐酸盐

Essential+ Grade
DAPI 4′,6-Diamidine-2′-phenylindole dihydrochloride

10236276001

DAPI

-
fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA, λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

solubility

DMF: soluble, H2O: soluble

solubility

water: soluble

solubility

H2O: 20 mg/mL (Heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.), PBS: insoluble

solubility

-

form

powder

form

liquid

form

powder

form

powder

storage temp.

room temp

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

room temp

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

应用

在琼脂糖凝胶中染色DNA,DAPI比溴化乙锭敏感几倍。它可用于DNA的光足迹图谱,通过对双链复合体的特殊可视化检测印迹应用中的退火探针,并使用流式细胞分析术研究DNA中的变化并分析凋亡过程中的DNA含量。DAPI染色也是一种敏感和特异的支原体检测方法。

生化/生理作用

DNA的细胞渗透性荧光小沟结合探针。与双链DNA的小沟(富含AT的DNA优先)结合,形成稳定的复合物,其发出的荧光比单独的DAPI高约20倍。

包装

无底玻璃瓶。内含物在插入的融合锥体内。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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